UVA molecular electron microscopy core for the Mid-Atlantic region

大西洋中部地区的 UVA 分子电子显微镜核心

• 批准号: 9548722
• 负责人: Vitold Galkin
• 金额: $ 32.3万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9548722
• 关键词: Biological Sciences; Collaborations; Communication; Core Facility; Cryoelectron Microscopy; Data; Data Collection; Electron Microscope; Electron Microscopy; Electrons; Faculty; Floor; Fostering; Freezing; Funding; Future; Grant; Growth; Image Analysis; Laboratories; Lighting; Macromolecular Complexes; Mid-Atlantic Region; Molecular; Molecular Medicine; Montserrat; Productivity; Research; Research Infrastructure; Resolution; Robotics; Schools; Scientist; Students; Time; Titan; Translational Research; United States National Institutes of Health; Universities; Virginia; Vision; Visit; design; detector; experience; improved; instrumentation; lens; medical schools; member; structural biology; time use; transmission process

Abstract The Molecular Electron Microscopy Core (MEMC) facility at the University of Virginia (UVa) is comprised of three transmission electron microscopes. An FEI 120 kV Spirit is housed on the second floor of the Snyder Translational Research Building at Fontaine Research Park. An FEI 200 kV F20 and a 300 kV Titan Krios are located in a specially designed suite in the underground Life Sciences Annex (LiSA) adjacent and connected to the Snyder Building. Anticipating future growth in high-resolution cryoEM research, the suite was designed to accommodate two Titan Krios electron microscopes. The Titan Krios has an autoloader that robotically manipulates up to 12 frozen grids inserted at one time, uses constant current lenses for greater stability, is equipped with an XFEG for improved illumination and a Falcon II direct electron detector. In 2009, the abstract of our G20 grant that supported the build-out of the LiSA suite stated, “The MEMC facility will (1) enhance the quality of research at UVa, (2) increase scientific productivity, (3) foster communication, interactions and collaboration amongst scientists, (4) boost the ability of UVa to attract and maintain premier faculty and students, and (5) strengthen the competitiveness of our faculty for funding. The MEMC facility will not only be available to School of Medicine faculty members but also to other scientists in other schools and departments at UVa. This will be a unique Core laboratory at UVa, and we envision that scientists throughout Virginia and the surrounding states will visit UVa to take advantage of such a cutting-edge laboratory for performing molecular resolution electron microscopy.” We have a strong spirit of collegiality and collaboration at UVa and welcome the opportunity to share the MEMC facility within UVa and beyond. We believe that our vision of the MEMC facility as a state-of-the-art cryoEM center for scientists in the mid-Atlantic region has already been realized. Over the last two years, 23 labs at UVa and 12 external labs have collected data using the MEMC. 14 labs have collected data using the Titan Krios. Half of the available time will be devoted to Consortium PIs, who have substantial experience in cryoEM and image analysis: Drs. Jenny Hinshaw (NIH), Deborah Kelly (Virginia Tech), Melanie Ohi (Vanderbilt), Montserrat Samso (VCU), and Peijun Zhang (Pitt). As the primary internal UVa users of the MEMC, Drs. Yeager and Egelman have more than 5 decades of experience. As Director of the Core, Dr. Kelly Dryden interacts closely with all users. The U24 grant, combined with $300K/year provided by the UVa School of Medicine, would make the MEMC financially sustainable.

抽象的 弗吉尼亚大学（UVA）的分子电子显微镜核心（MEMC）设施已完成 三个透射电子显微镜。 FEI 120 KV精神被安置在Snyder的二楼 Fontaine研究园的翻译研究大楼。 Fei 200 kV F20和300 kV的泰坦krios是 位于地下生命科学附件（LISA）的特殊设计套房，相邻与 Snyder建筑物。该套件旨在预期高分辨率冷冻研究的未来增长，旨在 容纳两个泰坦krios电子显微镜。 Titan Krios有一个自动加载器 一次插入多达12个冷冻网格，使用恒定的电流镜头以提高稳定性，是 配备XFEG改善照明和Falcon II直接电子检测器。在2009年，抽象 支持Lisa Suite建立的G20赠款中说：“ MEMC设施将（1）增强 UVA的研究质量，（2）提高科学生产力，（3）促进交流，互动和 科学家之间的合作，（4）提高UVA吸引和维护高级教师的能力 学生和（5）加强我们教职员工在资金方面的竞争力。 MEMC设施不仅将是 医学院教职员工，也可用于其他学校和部门的其他科学家 在UVA。这将是UVA的独特核心实验室，我们设想整个弗吉尼亚州和 周围的州将访问UVA，以利用这样的尖端实验室进行表演 分子分辨率电子显微镜。“我们在UVA和 我们欢迎有机会在UVA及其他地区共享MEMC设施。我们相信我们对 MEMC设施是中大西洋地区的最先进的科学家中心 实现。在过去的两年中，UVA的23个实验室和12个外部实验室已使用MEMC收集了数据。 14个实验室已使用Titan Krios收集了数据。一半的可用时间将专门用于财团， 在冷冻和图像分析中具有丰富经验的人：Drs。珍妮·辛肖（NIH），黛博拉·凯利 （弗吉尼亚理工学院），梅兰妮·奥西（Melanie Ohi）（范德比尔特（Vanderbilt）），蒙特塞拉特·萨姆索（Montserrat Samso）（VCU）和Peijun Zhang（Pitt）。作为主要 MEMC的内部UVA用户Drs。 Yeager和Egelman拥有超过5年的经验。作为 核心主任凯利·德莱登（Kelly Dryden）博士与所有用户紧密互动。 U24赠款，结合 UVA医学院提供的30万美元/年将使MEMC在财务上可持续。