Minnesota HHEAR Targeted Analysis Laboratory

明尼苏达州 HHEAR 靶向分析实验室

• 批准号: 9813860
• 负责人: Lisa A Peterson
• 金额: $ 80万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9813860
• 关键词: Affect; Age; Analytical Chemistry; Area; Beverages; Biological; Biological Assay; Biological Markers; Blood; Carcinogens; Client; Clinical Research; Consultations; DNA Adducts; DNA analysis; Data; Data Analyses; Development; Diet; Dose; Eating; Environmental Exposure; Environmental Impact; Exposure to; Formalin; Goals; Hair; Health; Human; Infrastructure; International; Laboratories; Laboratory Scientists; Life Style; Link; Mass Spectrum Analysis; Measurement; Measures; Methods; Minnesota; Nutritional; Outcome; Plasma; Prevention strategy; Procedures; Quality Control; Research Design; Research Personnel; Resource Development; Resources; Role; Saliva; Sampling; Scientist; Stress; Structure of nail of toe; Talents; Testing; Time; Tissues; United States; Universities; Urine; Variant; base; design; disorder prevention; epidemiology study; experience; human disease; outreach; programs; tobacco exposure; toxicant

PROJECT SUMMARY/ABSTRACT: OVERALL The Minnesota HHEAR Targeted Analysis Laboratory brings together investigators from the University of Minnesota and the Minnesota Department of Health with broad and internationally recognized expertise in the identification and analysis of agents that are potentially harmful to human health at all ages. The combination of expertises in our proposed laboratory is unique. Our scientists have years of experience in the development and application of state-of-the-art high throughput analytical chemistry methods based mainly on mass spectrometry for the assessment of trace amounts of targeted toxicant or carcinogen metabolites in large clinical and epidemiological studies. Our scientists also have developed and applied methods for the analysis of DNA adducts of cancer-causing agents in human samples. There are few groups that can bring together a team with such superb and creative expertise on multiple relevant levels of exposure assessment. Therefore, our overall goal is to provide human health researchers with access to state-of-the-art infrastructure for targeted analysis of biological samples. We see this goal as firmly embedded within the concept of the exposome and its relationship to human health. The specific aims of each component resource and the development core are as follows. The Targeted Analysis Resource will use predominantly high throughput mass spectrometric methods with well-established quality control procedures to provide timely and reliable quantitative data relevant to 3 major areas of importance to human health: Exposure to tobacco-specific compounds; exposure to environmental, lifestyle, and nutritional toxicants and carcinogens; and variations in levels of endogenous electrophiles and dietary compounds. The Developmental Core will establish new high throughput methods to measure multiple classes of toxicants and biomarkers in urine, plasma, hair, and toenails; to detect nutritional biomarkers from the intake of foods and beverages in urine and plasma; to develop assays to detect a panel of internal biomarkers of stress in multiple biospecimens; and expand our approaches to detect DNA adducts in saliva, blood and formalin-fixed tissues. The Administrative Core will coordinate all activities within the Minnesota HHEAR Targeted Analysis Laboratory. In addition, this core will be the primary link to the HHEAR Coordinating and Data Centers as well as the HHEAR investigators at all stages of their projects. Collectively, the Minnesota HHEAR Targeted Analysis Laboratory will contribute to a productive and effective HHEAR network designed to understand the impact of environmental exposures on human health.

项目概要/摘要：总体 明尼苏达州 HHEAR 靶向分析实验室汇集了来自明尼苏达大学的研究人员 明尼苏达州和明尼苏达州卫生部在以下方面拥有广泛且国际公认的专业知识 识别和分析对所有年龄段的人类健康可能有害的物质。组合 我们提议的实验室的专业知识是独一无二的。我们的科学家拥有多年的开发经验 主要基于质量的最先进的高通量分析化学方法和应用 光谱分析法用于评估大量目标毒物或致癌物代谢物 临床和流行病学研究。我们的科学家还开发并应用了分析方法 人类样本中致癌物质的 DNA 加合物。很少有团体能够聚集在一起 团队在多个相关级别的暴露评估方面拥有如此精湛和富有创造力的专业知识。所以， 我们的总体目标是为人类健康研究人员提供最先进的基础设施 生物样本的针对性分析。我们认为这一目标已牢固地融入到“ 暴露组及其与人类健康的关系。每个组件资源的具体目标和 开发核心如下。 目标分析资源将主要使用高通量质谱方法 完善的质量控制程序，提供与 3 个主要相关的及时可靠的定量数据 对人类健康具有重要意义的领域：接触烟草特有的化合物；暴露于环境， 生活方式、营养毒物和致癌物；以及内源性亲电子试剂水平的变化和 膳食化合物。 发展核心将建立新的高通量方法来测量多类有毒物质 以及尿液、血浆、头发和脚趾甲中的生物标志物；检测食物摄入中的营养生物标志物 尿液和血浆中的饮料；开发检测一组压力内部生物标志物的方法 多个生物样本；并扩展我们检测唾液、血液和福尔马林固定液中 DNA 加合物的方法 组织。 行政核心将协调明尼苏达州 HHEAR 目标分析内的所有活动 实验室。此外，该核心也将成为 HHEAR 协调和数据中心的主要链接 作为项目各个阶段的 HHEAR 调查员。 总的来说，明尼苏达州 HHEAR 目标分析实验室将有助于建立富有成效的、有效的分析实验室。 HHEAR 网络旨在了解环境暴露对人类健康的影响。