Development of Reagents for Sequencing Proteins and Protein Fragments on a Next-Generation DNA Sequencer

开发用于在下一代 DNA 测序仪上对蛋白质和蛋白质片段进行测序的试剂

• 批准号: 9679623
• 负责人: KEVIN L GUNDERSON
• 金额: $ 71.83万
• 依托单位: ENCODIA, INC.
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-05 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9679623
• 关键词: Adoption; Applied Research; Area; Automation; Basic Science; Binding; Bioinformatics; Biological; Biological Assay; Biological Sciences; Chemistry; Clinical Research; Code; Complex; Computer software; DNA; DNA Sequence; Development; Drug Targeting; Enzymes; Event; Generations; Genomics; Goals; Immobilization; Industry; Label; Length; Ligation; Maps; Methods; Modeling; Molecular; Peptide Sequence Determination; Peptides; Phase; Polymerase; Preparation; Protein Analysis; Protein Engineering; Protein Fragment; Proteins; Proteomics; Reagent; Sampling; Signal Transduction; Solid; Surface; System; Technology; Titrations; Trypsin; Writing; cost; digital; drug development; high throughput analysis; improved; innovation; next generation; next generation sequencing; optical imaging; single molecule; usability; user-friendly

ABSTRACT Next generation sequencing (NGS) has transformed the field of genomics and enabled broad adoption of the technology in biological, translational, and most recently clinical research. The proteomics equivalent to NGS has not yet been realized. As such, the goal of this proposal is to develop a highly-scalable digital protein analysis technology. The approach is broadly applicable and greatly improves upon cost, sensitivity, usability, and throughput of current approaches.

抽象的 下一代测序（NGS）改变了基因组学领域并启用了广泛 在生物，转化和最近的临床研究中采用了该技术。这 等效于NGS的蛋白质组学尚未实现。因此，该提议的目标是 开发高度可观的数字蛋白质分析技术。该方法广泛适用，并且 当前方法的成本，敏感性，可用性和吞吐量大大提高了。