ShEEP Request for Laser Scanning Confocal Microscopy Acquisition and Analysis System

ShEEP 请求激光扫描共焦显微镜采集和分析系统

• 批准号: 9795294
• 负责人: Richard Altschuler
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795294
• 关键词: 3-Dimensional; Affect; Aging; Air; Algorithms; Anatomy; Applications Grants; Award; Biopsy Specimen; Central Nervous System Infections; Chronic Obstructive Airway Disease; Chronic lung disease; Cochlea; Collaborations; Computer software; Confocal Microscopy; Data; Data Analyses; Data Collection; Detection; Disease; Effectiveness; Elements; Equilibrium; Equipment; Faculty; Firearms; Fluorescence Resonance Energy Transfer; Funding; Future; Glycerol; Golf; Grant; Healthcare Systems; Hearing; Height; Image; Image Analysis; Image Enhancement; Imagery; Immersion Investigative Technique; Immunotherapy; In Situ; Individual; Infection; Label; Labyrinth; Laser Scanning Confocal Microscopy; Lasers; Learning; Measurement; Michigan; Microscope; Molecular; Nerve Degeneration; Neurodegenerative Disorders; Noise; Normal tissue morphology; Oils; Organ; Pathogenesis; Pathologic; Pathologic Processes; Pathology; Patients; Population; Post-Traumatic Stress Disorders; Preparation; Process; Research; Research Institute; Research Personnel; Resolution; Rotation; Running; Scanning; Services; Sheep; Site; Specimen; Surface; System; Systems Analysis; Testing; Therapeutic Intervention; Thick; Three-dimensional analysis; Time; Tissue Preservation; Tissues; Toxic effect; Toxicant exposure; Training; United States National Institutes of Health; Universities; Veterans; War; Water; cancer type; chromophore; design; detector; experience; hearing impairment; high resolution imaging; image visualization; improved; live cell imaging; neoplasm immunotherapy; ranpirnase; response; ribbon synapse; systems research; targeted treatment; tissue repair; tumor growth; 3-Dimensional; Affect; Aging; Air; Algorithms; Anatomy; Applications Grants; Award; Biopsy Specimen; Central Nervous System Infections; Chronic Obstructive Airway Disease; Chronic lung disease; Cochlea; Collaborations; Computer software; Confocal Microscopy; Data; Data Analyses; Data Collection; Detection; Disease; Effectiveness; Elements; Equilibrium; Equipment; Faculty; Firearms; Fluorescence Resonance Energy Transfer; Funding; Future; Glycerol; Golf; Grant; Healthcare Systems; Hearing; Height; Image; Image Analysis; Image Enhancement; Imagery; Immersion Investigative Technique; Immunotherapy; In Situ; Individual; Infection; Label; Labyrinth; Laser Scanning Confocal Microscopy; Lasers; Learning; Measurement; Michigan; Microscope; Molecular; Nerve Degeneration; Neurodegenerative Disorders; Noise; Normal tissue morphology; Oils; Organ; Pathogenesis; Pathologic; Pathologic Processes; Pathology; Patients; Population; Post-Traumatic Stress Disorders; Preparation; Process; Research; Research Institute; Research Personnel; Resolution; Rotation; Running; Scanning; Services; Sheep; Site; Specimen; Surface; System; Systems Analysis; Testing; Therapeutic Intervention; Thick; Three-dimensional analysis; Time; Tissue Preservation; Tissues; Toxic effect; Toxicant exposure; Training; United States National Institutes of Health; Universities; Veterans; War; Water; cancer type; chromophore; design; detector; experience; hearing impairment; high resolution imaging; image visualization; improved; live cell imaging; neoplasm immunotherapy; ranpirnase; response; ribbon synapse; systems research; targeted treatment; tissue repair; tumor growth

This proposal is for a shared Laser Scanning Confocal Microscopy Acquisition and Analysis System to allow a Major User group (six Merit Award funded PIs) as well as other VA researchers at the VA Ann Arbor Health Care System (VAAAHS) to conduct high-resolution analysis in both living and preserved tissues. Research focuses on cellular pathogenesis of diseases that preferentially affect veteran populations. Studies include firearm triggered loss of hearing and balance, Deployment-Related Chronic Lung Disease, PTSD, COPD, CNS infections and subset of neurodegenerative disorders, certain types of cancer (tumor immunotherapy). These diseases are characterized by specific, and often subtle, micro-anatomic tissue changes and a detailed identification and assessment at the molecular level is critical to execute studies, propose new treatment opportunities and subsequently test their effectiveness. This need is not being adequately met. The requested system is composed of Leica SP-8 inverted laser scanning confocal microscope with a Tokia HIT stage-top incubation system for live cell imaging. It will have multiple lasers and high capability detectors with adjustable channels, rapid acquisition capacity and up to 120 nm resolution. Air, water, oil and glycerol immersion objectives with varying working distances and numerical apertures will match the needs of our diverse user group for their variety of specimens and assessments. Software on both acquisition and image analysis workstations will provide for enhanced imaging, visualization, co-localization and rotation as well as 2-D and 3- D analysis and measurement. FRET and FRAP “wizards” will allow these applications. Introducing new capabilities will enhance current studies by providing new data and improved data collection and analyses as well as increasing efficiency and throughput. It will extend current studies into new directions by allowing identifications and assessments that would not otherwise be possible, for example, by allowing new multiple co-labeling of molecules and cellular elements deep into specimens or by allowing real-time dynamic live cell imaging, FRET and FRAM analyses. It will also create new capabilities aiding future grant applications of our faculty and new learning opportunities for trainees at the VAAAHS site. The requested system will enhance and extend VAAAHS research on disorders effecting our veteran population towards developing therapeutic interventions to ultimately benefit our patients.

该建议是针对共享激光扫描共聚焦显微镜采集和分析系统，以允许 主要用户组（六项优异奖资助的PI）以及VA Ann Arbor Health的其他VA研究人员 护理系统（VAAAHS）在生物和保存组织中进行高分辨率分析。研究 着重于优先影响退伍军人人群的疾病的细胞发病机理。研究包括 枪支引发了听力和平衡的丧失，与部署相关的慢性肺病，PTSD，COPD，CNS 神经退行性疾病的感染和子集，某些类型的癌症（肿瘤免疫疗法）。这些 疾病的特征是特定的，通常是微妙的微动物组织变化，详细的 分子水平的识别和评估对于执行研究至关重要，提出新的治疗方法 机会并随后测试其有效性。这种需求无法得到充分满足。请求 系统由Leica SP-8倒激光扫描共聚焦显微镜组成 活细胞成像的孵育系统。它将具有多个激光器和高功能检测器，可调节 渠道，快速获取能力和最高120 nm的分辨率。空气，水，油和甘油浸入 具有不同工作距离和数值孔径不同的目标将符合我们多元化用户的需求 组以各种标本和评估。有关获取和图像分析的软件 工作站将提供增强的成像，可视化，共定位和旋转以及2-D和3-- D分析和测量。 FRET和FRAP“向导”将允许这些应用程序。引入新 功能将通过提供新的数据并改进数据收集和分析作为作为当前研究的功能。 以及提高效率和吞吐量。它将通过允许将当前研究扩展到新的方向 例如，通过允许新的倍数来进行识别和评估 分子和细胞元件的共标记深入标本，或通过允许实时动态活细胞 成像，FRET和FRAM分析。它还将创建新的功能，以帮助我们的未来赠款应用 VAAAHS网站的学员的教师和新的学习机会。请求的系统将增强 并扩展了有关疾病的VAAAHS研究，从而影响我们的老兵发展治疗 干预措施最终使我们的患者受益。