Myo10-Driven Filopodia in Skeletal Muscle

骨骼肌中 Myo10 驱动的丝状伪足

• 批准号: 9795646
• 负责人: H Lee Sweeney
• 金额: $ 37.6万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795646
• 关键词: Actins; Acute; Age; Blood Vessels; Breathing; Cell Culture Techniques; Cell Nucleus; Cell fusion; Cells; Characteristics; Chimeric Proteins; Chronic; Data; Defect; Development; Direct Lytic Factors; Disease; Duchenne muscular dystrophy; Female; Fiber; Filopodia; Freezing; Genetic; Growth; Human; In Vitro; Injury; Knock-out; Laboratories; Locomotion; Mammals; Modeling; Molecular Motors; Motor; Movement; Mus; Muscle; Muscle Cells; Muscle Development; Muscle Fibers; Muscle satellite cell; Myoblasts; Myopathy; Myosin ATPase; Natural regeneration; Nature; Neoplasm Metastasis; Phenotype; Physiological; Play; Probability; Process; Proteins; Regulation; Role; Sarcomeres; Skeletal Muscle; Skeleton; Stem cells; Tissues; experimental study; in vivo; in vivo regeneration; insight; male; muscle regeneration; postnatal; relating to nervous system; repaired; satellite cell; skeletal muscle growth

Myo10-Driven Filopodia in Skeletal Muscle: H. Lee Sweeney, P.I. Abstract: This project will investigate the role of an unconventional myosin, myosin X (Myo10), and the filopodia that it generates in myoblasts and nascent myotubles. We postulate that it is only present in muscle cells/satellite cells that are actively in the process of fusing with each other, which would be during muscle growth and regeneration. We further postulate that Myo10 forms filopodia and carries components of the fusion machinery to the tips of those filipodia, greatly increasing the efficiency and probability of cell fusion. We will examine the consequences of loss of Myo10 and its interactions in myogenic cells in the following aims: 1) Delineate the role of myosin X (Myo10) involvement in myoblast fusion and identify regulatory mechanisms of protein targeting and activation in vitro; 2) Evaluate the requirement of myosin X (Myo10) for muscle regeneration; and, 3) Investigate Myosin X (Myo10) as a modifier of chronic muscle disease. These experiments will uncover previously unstudied aspects of the fusion process for skeletal muscle growth and regeneration, and may delineate new modifiers/targets in disease states of skeletal muscle that require rapid muscle regeneration using satellite cells.

骨骼肌中的Myo10驱动丝状丝虫：H。Lee Sweeney，P.I。 抽象的： 该项目将调查非常规肌球蛋白，肌球蛋白X（Myo10）和丝状肌的作用 生成肌细胞和新生的肌表演。我们假设它仅存在于肌肉细胞/卫星细胞中 这是在彼此融合的过程中积极的，这将是在肌肉生长和再生过程中。 我们进一步假设Myo10形成了丝状曲霉，并将融合机械的组件载于 那些菲律宾人大大提高了细胞融合的效率和概率。我们将检查后果 在以下目的中，Myo10损失及其在肌原细胞中的相互作用：1）描绘肌球蛋白X的作用 （myo10）参与成肌细胞融合并确定蛋白质靶向和激活的调节机制 体外; 2）评估肌球蛋白X（MyO10）对肌肉再生的需求； 3）研究肌球蛋白X （myo10）作为慢性肌肉疾病的修饰符。这些实验将发现以前未研究的方面 骨骼肌生长和再生的融合过程，并可能描绘出新的修饰符/目标 需要使用卫星细胞快速肌肉再生的骨骼肌疾病状态。