Expanding the toolbox for tsetse reproductive biology

扩展采采蝇生殖生物学的工具箱

基本信息

批准号：
8622915
负责人：
Serap AKSOY
金额：
$ 24.17万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-01-06 至 2015-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8622915
关键词：
Affect Africa South of the Sahara African Trypanosomiasis Animal Model Animals Behavior Behavioral Biology Complex Computer Simulation Data Data Set Deposition Development Disease Disease Vectors Disease model Evolution Female Fertility Fluorescent in Situ Hybridization Foundations Future Gene Expression Gene Expression Profile Gene Proteins Gene Silencing Genome Genomics Gland Goals Human Insecta Knowledge Longevity Mediating Methods Molecular Genetics Molecular Profiling Nucleic Acid Regulatory Sequences Oogenesis Organism Orthologous Gene Partner in relationship Pattern Pesticides Physiological Physiology Play Polymorphism Analysis Population Population Sizes Proteins Proteomics Protocols documentation Regulation Regulatory Element Reproduction Reproductive Biology Reproductive Physiology Research Resources Role Sampling Seminal fluid Set protein Specificity Spermatogonia Study models Synteny Techniques Tissues Toxic effect Trypanosomiasis Tsetse Flies base comparative cost disease transmission egg fitness fly knowledge base male neglect non-genetic novel offspring paralogous gene prevent protein function public health relevance reproductive sex sperm cell tool transcriptomics transmission process vector control young adult

项目摘要

Project Summary/Abstract Tsetse flies transmit the human and animal forms of African Trypanosomiasis, neglected diseases, which affect marginalized populations in sub-Saharan Africa. Control methods based upon pesticide-baited targets and traps can reduce tsetse populations, but have downsides, including environmental toxicity, collateral effects upon non- target organisms and high implementation cost. Thus, genetic and non-pesticide based vector control methods are desirable. This project will build the foundation for control methods that aim to reduce tsetse's reproductive capacity. We will investigate male accessory gland (MAGs) products, known as seminal fluid proteins (SFPs), which are transferred along with sperm to the female during mating. SFPs mediate important physiological and behavioral changes in mated female insects, including reduced receptivity to remating and increased oogenesis/egg deposition. Disruption/manipulation of SFP function(s) has the potential to reduce tsetse populations and hence disease transmission. Tsetse flies have a low reproductive capacity as they reproduce by obligate viviparity. Each gonotrophic cycle, females carry and nourish a single intrauterine offspring for the duration of its development. Females on average produce 8-10 offspring per lifetime. Our proposed studies are supported by the availability of 1) preliminary transcriptomic data on MAG gene expression in our target species Glossina morsitans morsitans (Gmm), 2) an annotated genome and extensive transcriptomic resources for Gmm, and 3) a large dataset of transcriptomes from other tsetse species. This study has 3 aims: 1. Identify putative SFP genes using transcriptomic, proteomic and genomic approaches, characterize their sex/tissue specificity, and examine temporal expression profiles during maturation and after mating. 2. Identify SFP orthologs and paralogs between multiple Glossina species by utilizing available transcriptomic and genomic resources, estimate sequence diversity of SFPs by polymorphism detection and identify conserved sex and tissue specific regulatory elements by in silico analysis. 3. Develop and apply gene silencing techniques to assess the functional role(s) of selected SFPs (~5) within the context of the tsetse reproductive fitness parameters.

项目概要/摘要采采蝇传播人类和动物形式的非洲锥虫病，这是一种被忽视的疾病，影响撒哈拉以南非洲的边缘化人口。基于农药诱饵目标和陷阱的控制方法可以减少采采蝇数量，但也有缺点，包括环境毒性、对非目标生物体和实施成本高。因此，基于遗传和非农药的病媒控制方法是可取的。该项目将为旨在减少采采蝇繁殖的控制方法奠定基础容量。我们将研究男性附属腺 (MAG) 产品，称为精液蛋白 (SFP)，在交配过程中与精子一起转移到雌性体内。 SFP 介导重要的生理和交配雌性昆虫的行为变化，包括减少重新交配的接受度和增加卵子发生/卵沉积。破坏/操纵 SFP 功能有可能减少采采蝇人口和疾病传播。采采蝇的繁殖能力较低，因为它们通过强制胎生。每个生殖周期，雌性都会携带并滋养一个子宫内后代其发展的持续时间。女性一生平均生育 8-10 个后代。我们提出的研究是 1) 我们目标物种中 MAG 基因表达的初步转录组数据的支持 Glossina morsitans morsitans (Gmm)，2) Gmm 的注释基因组和广泛的转录组资源， 3）来自其他采采蝇物种的转录组的大型数据集。这项研究有 3 个目标： 1. 使用转录组学、蛋白质组学和基因组学方法鉴定假定的 SFP 基因，描述其特征性别/组织特异性，并检查成熟期间和交配后的时间表达谱。 2. 利用现有的转录组学鉴定多个 Glossina 物种之间的 SFP 直向同源物和旁系同源物和基因组资源，通过多态性检测估计SFP的序列多样性并鉴定通过计算机分析保守的性别和组织特异性调节元件。 3. 开发并应用基因沉默技术来评估所选 SFP (~5) 在采采蝇繁殖适应性参数的背景。