In Vivo Measurements of Physiologically-evoked Neuronal Currents

生理诱发神经元电流的体内测量

• 批准号: 8385726
• 负责人: JIA-HONG GAO
• 金额: $ 19.19万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8385726
• 关键词: Animal Model; Blood; Brain; Brain imaging; Characteristics; Cognitive; Communities; Complex; Copper; Coupling; Detection; Development; Electroencephalography; Event; Foundations; Functional Magnetic Resonance Imaging; Future; Goals; Hemocyanin; Hemoglobin; Human; Image; Imaging Techniques; Lead; Left; Lobe; Magnetic Resonance Imaging; Mammals; Maps; Measurable; Measurement; Measures; Mental disorders; Metabolism; Methods; Motor; Nature; Neurons; Neurosciences; Neurosciences Research; Octopus; Optics; Oxygen; Population; Positron-Emission Tomography; Process; Property; Reporting; Research Personnel; Research Project Grants; Resolution; Retina; Sensory; Signal Transduction; Source; Synapses; System; Techniques; Technology; Time; Transportation; Uncertainty; Variant; Visual system structure; base; blood oxygen level dependent; hemodynamics; human subject; improved; in vivo; innovation; innovative technologies; nervous system disorder; neuroimaging; regenerative; research study; success; tool

DESCRIPTION (provided by applicant): The goal of this project is to use octopus as an in vivo animal model to explore the ability of MRI to directly detect the neuronal electrical currents evoked by normal sensory stimulation. In principle, neuronal current MRI (ncMRI) offers improved spatio-temporal resolution over current hemodynamic-based functional MRI methods. However, contradictory reports regarding the detection of ncMRI signal in human subjects have left the feasibility of ncMRI an open question. Since most previous human ncMRI studies are problematic due to their inability to separate or eliminate hemodynamic effects, no conclusion is yet been possible whether ncMRI is achievable. To resolve this long-standing debate convincingly, we will detect visually evoked ncMRI signals in the octopus. The octopus has a large brain and a highly developed visual system, but its blood does not produce any blood oxygen level dependent (BOLD) contrast. Therefore, it is an ideal in vivo animal model for measuring ncMRI without BOLD contamination. The proposed ncMRI is an innovative technology that aims to image neuronal electrical currents directly. It should have a high impact by connecting the electrophysiological events in neurons with the systems-level view of large neuronal populations afforded by conventional MRI techniques. Successful development of this proposed research project will resolve the long-standing debate on the feasibility of ncMRI. Specifically, detection of any ncMRI signal will enable us, for the first time, to describe the temporal and spatial characteristics of neuronal current imaging. These measurements, in turn, will provide the necessary foundation for acquisition strategies and interpretations of future human ncMRI experiments. PUBLIC HEALTH RELEVANCE: The aim of this proposal is to explore the feasibility of using MRI to detect neuronal electrical currents directly. Success in mapping of neuronal currents using MRI will change the way brain imaging is done in neuroscience research, including the study of neurological and psychiatric disorders.

描述（由申请人提供）：该项目的目的是将章鱼用作体内动物模型，以探索MRI直接检测正常感觉刺激引起的神经元电流的能力。原则上，神经元电流MRI（NCMRI）比基于血液动力学的功能MRI方法提供了改进的时空分辨率。但是，关于人类受试者中NCMRI信号检测的矛盾报告使NCMRI的可行性成为一个空旷的问题。由于大多数以前的人类NCMRI研究由于无法分离或消除血液动力学作用而存在问题，因此是否可以实现NCMRI，尚无结论。为了令人信服地解决这一长期存在的辩论，我们将在章鱼中检测到视觉唤起的NCMRI信号。章鱼的大脑和高度发达的视觉系统，但其血液不会产生任何依赖性血氧水平（粗体）对比度。因此，它是用于测量NCMRI而无需大胆污染的理想体内动物模型。拟议的NCMRI是一种创新技术，旨在直接对神经元电流进行成像。它应该通过将神经元的电生理事件与传统MRI技术提供的大型神经元种群的系统级别的视图联系起来，从而产生很大的影响。该拟议的研究项目的成功发展将解决有关NCMRI可行性的长期辩论。具体而言，对任何NCMRI信号的检测都将使我们首次能够描述神经元电流成像的时间和空间特征。反过来，这些测量将为未来人类NCMRI实验的获取策略和解释提供必要的基础。 公共卫生相关性：该提案的目的是探索使用MRI直接检测神经元电流的可行性。使用MRI映射神经元电流的成功将改变神经科学研究中的大脑成像的方式，包括研究神经和精神疾病。