in situ Epigenetic Profiling of Single Cells in Kidney - Kim Diversity Supplement 2023

肾脏单细胞原位表观遗传分析 - Kim Diversity Supplement 2023

基本信息

批准号：
10849346
负责人：
Joshua Vaughan
金额：
$ 5.8万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-08-01 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10849346
关键词：
Address Adult Aging American Blood Cell Separation Cells Cessation of life Chromatin Chronic Kidney Failure Complex Correlative Study Cultured Cells DNA DNA Methylation Dialysis procedure Disease Dissociation Electrolyte Balance End stage renal failure Endothelial Cells Epigenetic Process Evaluation Face Fluorescent in Situ Hybridization Generations Genome Goals Health Expenditures Heterochromatin Histones Image Immunofluorescence Immunologic In Situ Individual Injury Kidney Kidney Diseases Label Maps Measures Methods Microscopy Molecular Molecular Conformation Mus Organ Phenotype Physiological Physiology Population Post-Translational Protein Processing Publishing Renal function Research Personnel Risk Role Specimen Stains Techniques Time Tissues Transplantation Use of New Techniques Work base blood pressure regulation epigenetic profiling field study genome-wide genomic locus glomerular basement membrane healthy aging histone modification human old age (65+)innovation insight kidney cell kidney imaging molecular phenotype mouse model nanoscale novel podocyte senescence single cell analysis superresolution imaging superresolution microscopy tool ultra high resolution wasting whole genome

项目摘要

Project Summary/Abstract We have recently developed a microscopy-based method that, for the first time, enables interrogation of histone modifications at single loci in single cells. This method is termed SCEPTRE (for Single-Cell Evaluation of Post TRanslational Epigenetic encoding) and utilizes super-resolution imaging to directly resolve and quantify histone modifications at specific DNA or gene loci that are labeled by immunofluorescence and DNA fluorescence in situ hybridization (FISH), respectively. We propose to develop SCEPTRE, a method currently in use with cultured or isolated cells, for epigenetic profiling of kidney tissues and to use SCEPTRE to study healthy aging and senescence in mouse kidney tissues.

项目概要/摘要我们最近开发了一种基于显微镜的方法，首次能够对组蛋白进行询问单细胞中单个基因座的修饰。该方法被称为 SCEPTER（用于后处理的单细胞评估）表观遗传翻译编码）并利用超分辨率成像直接解析和量化组蛋白通过免疫荧光和 DNA 荧光原位标记的特定 DNA 或基因位点的修饰杂交（FISH），分别。我们建议开发 SCEPTRE，这是一种目前用于培养或分离的细胞，用于肾组织的表观遗传分析，并使用 SCEPTER 研究健康衰老和小鼠肾组织的衰老。