Multi-scale Spatial Mapping of Human Lymphatic Vessels

人体淋巴管的多尺度空间测绘

• 批准号: 10887817
• 负责人: Young-Kwon Hong
• 金额: $ 19.99万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-25 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10887817
• 关键词: 3-Dimensional; Affect; Anatomy; Animal Model; Bioethics; California; Cancer Survivorship; Clinical; Collaborations; Communication; Communities; Continuance of life; Data; Development; Ensure; Future; Generations; Goals; Grant; Health; Human; Human BioMolecular Atlas Program; Human Resources; Indiana; Individual; Infrastructure; Institution; Israel; Knowledge; Leadership; Logistics; Lymph Node Mapping; Lymphatic; Lymphatic Capillaries; Lymphatic Diseases; Lymphatic System; Lymphedema; Lymphoid Tissue; Maps; Medical center; Mission; Operative Surgical Procedures; Patients; Pennsylvania; Procedures; Process; Reproducibility; Research Personnel; Resource Sharing; Resources; Sampling; Specimen; Structure; Therapeutic; Three-Dimensional Imaging; Tissues; United States; United States National Institutes of Health; Universities; Visualization; data dissemination; data integration; data sharing; data submission; lymphatic imaging; lymphatic vessel; pharmacologic; programs; radiological imaging; tissue mapping; transcriptome; transcriptomics

Abstract: Lymphatic disease affects over 10 million patients in the United States and are grossly understudied. Lymphedema, the most commonly occurring lymphatic disease, is considered one on of the most significant cancer survivorship issues in the United States. Of significant note, our primary understanding and mapping of the lymphatic system come from animal models. A comprehensive anatomic reference from the human lymphatic system does not currently exist. We propose the development of a lymphatic tissue mapping center (TMC) as a part of the NIH Human Biomolecular Atlas Program (HuBMAP). Specifically, through a consortium of institutions including the Beth Israel Deaconess Medical Center, University of Southern California, University of Pennsylvania, Stanford University, and Indiana University, we will acquire both lymphatic collectors and lymphatic capillaries in healthy individuals across the entire health spectrum and register each of these samples to the associated angiosome thereby allowing coordination with HuBMAP. These samples will undergo spatial transcriptome analysis. Moreover, we will perform 3D radiologic imaging of lymphatic collectors throughout the body. The spatial transcriptome analysis and 3D imaging data will be processed and transferred to the HuBMAP Integration, Visualization, and Engagement (HIVE) Collaborative. The lymphatic tissue mapping center, alongside HuBMAP, will provide fine detail anatomic precision of the lymphatic system better informing future surgical interventions. The spatial transcriptome analysis will provide potential targets for future pharmacologic therapeutics.

抽象的： 在美国，淋巴疾病影响着超过 1000 万患者，并且严重影响 待研究。淋巴水肿是最常见的淋巴疾病，被认为是其中之一 美国最重要的癌症生存问题。值得注意的是，我们的主要理解 淋巴系统的图谱来自动物模型。全面的解剖学参考 人类淋巴系统目前还不存在。我们建议发展淋巴组织 绘图中心 (TMC) 作为 NIH 人类生物分子图谱计划 (HuBMAP) 的一部分。具体来说， 通过包括贝斯以色列女执事医疗中心、以色列大学在内的机构联盟 我们将收购南加州大学、宾夕法尼亚大学、斯坦福大学和印第安纳大学 整个健康范围内健康个体的淋巴收集器和毛细淋巴管， 将这些样本中的每一个注册到相关的血管体，从而允许与 HuBMAP。这些样本将进行空间转录组分析。此外，我们还将进行3D 全身淋巴管的放射成像。空间转录组分析和 3D 成像数据将被处理并传输到 HuBMAP 集成、可视化和参与 （蜂巢）协作。淋巴组织绘图中心与 HuBMAP 一起将提供精细的细节 淋巴系统的解剖精度更好地为未来的手术干预提供信息。空间 转录组分析将为未来的药物治疗提供潜在的靶点。

项目成果

Edematous Dermal Thickening on Magnetic Resonance Imaging as a Biomarker for Lymphatic Surgical Outcomes.

磁共振成像上的水肿性真皮增厚作为淋巴手术结果的生物标志物。

• 发表时间: 2023-07-26
• 作者: Kinney, JacqueLyn R;Babapour, Sara;Kim, Erin;Friedman, Rosie;Singhal, Dhruv;Lee, Bernard T;Tsai, Leo L
• 通讯作者: Tsai, Leo L