Cell Imaging Core

细胞成像核心

• 批准号: 8381654
• 负责人: MICHAEL H NATHANSON
• 金额: $ 16.16万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8381654
• 关键词: Biological; Calcium; Cells; Confocal Microscopy; Core Facility; DUSP1 gene; Data Analyses; Detection; Development; DsRed; Dyes; Equipment; Fatty Liver; Fluorescence Resonance Energy Transfer; Head; Human Resources; Image; Image Analysis; Immunofluorescence Immunologic; Lasers; Life; Liver; MAP Kinase Gene; Mediating; Microscope; Microscopy; Molecular; Nuclear; Optics; Organelles; Physics; Physiologic pulse; Proteins; Regulation; Research Personnel; Research Support; Role; Sapphire; Scanning; Services; Signal Transduction; Specialist; Specimen; System; Technical Expertise; Time; Titania; Titanium; Training; cell fixing; cellular imaging; complement system; detector; experience; flash photolysis; fluorescence imaging; fluorophore; image processing; medical schools; member; molecular imaging; multi-photon; research study

The purpose of the Cell Imaging Core (Core C) is to provide access to equipment and technical expertise for confocal microscopy, combined confocal and multi-photon microscopy, and image processing and analysis. The range of experiments that will take advantage of this core include immunofluorescence, imaging of GFP, DsRed, RFP, and other organelle- and protein-specific dyes, time-lapse confocal microscopy, calcium imaging, and confocal imaging combined with multi-photon excitation for photo-activation of dyes and flash photolysis of caged compounds. Core C will take advantage of the medical school's existing Center for Cell and Molecular Imaging in order to provide these services. Equipment in the core will include two Zeiss LSM 510 confocal microscopes, a Zeiss LSM 710 NLO Duo confocal/multiphoton microscope, and two PCs for off-line image processing and data analysis. The first of the three confocal microscopes is a standard pointscanning system with three detection channels, while the second is equipped with a META detector for spectral deconvolution, and the third has dual scan heads, nonlinear optics, non-descanned detectors, and is equipped with a Spectra-Physics 10 watt tunable femtosecond-pulsed titanium:sapphire laser for multiphoton excitation. Core C also will provide a Research Support Specialist who is experienced in confocal and multi-photon microiscopy. The Research Support Specialist will train members of each project in the use of the Core's equipment and also will be available for ongoing assistance with all experiments requiring the use of this core facility.e ascertained. Collectively, these studies will synergize with Projects 1 and 2 to define the role of nuclear Ca2+ and MKP-1-mediated MAPK signaling in the development of hepatic steatosis.

细胞成像核心（核心 C）的目的是提供共焦显微镜、共焦和多光子组合显微镜以及图像处理和分析的设备和技术专业知识。 利用该核心的实验范围包括免疫荧光、GFP、DsRed、RFP 和其他细胞器和蛋白质特异性染料的成像、延时共聚焦显微镜、钙成像以及与多光子激发相结合的共聚焦成像用于染料的光活化和笼状化合物的闪光光解。 Core C 将利用医学院现有的细胞和分子成像中心来提供这些服务。核心设备包括两台 Zeiss LSM 510 共焦显微镜、一台 Zeiss LSM 710 NLO Duo 共焦/多光子显微镜以及两台用于离线图像处理和数据分析的 PC。三个共焦显微镜中的第一个是标准点扫描 系统具有三个检测通道，第二个配备用于光谱反卷积的 META 探测器，第三个具有双扫描头、非线性光学、非反扫描探测器，并配备 Spectra-Physics 10 瓦可调谐飞秒脉冲钛：用于多光子激发的蓝宝石激光器。 Core C 还将提供一名在共焦和多光子显微术方面经验丰富的研究支持专家。研究支持专家将对每个项目的成员进行使用方面的培训 的核心设备，并且还将为需要使用该核心设施的所有实验提供持续的协助。总的来说，这些研究将与项目 1 和 2 协同作用，以确定核 Ca2+ 和 MKP-1 介导的 MAPK 信号在肝脂肪变性发展中的作用。