Administrative Diversity Supplement for Ta-Tyonna Buck.

Ta-Tyonna Buck 的行政多元化补充。

基本信息

批准号：
10841785
负责人：
Lakiesha Nicole Williams
金额：
$ 7.66万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2027-07-31
项目状态：
未结题

项目摘要

Project Summary: Cranial dura mater is disrupted during invasive brain surgeries, resulting in major post-surgical complications that may be fatal if left untreated. Commercial dura grafts perform sub-optimally, creating a critical need for a dura graft substitute that maintains structural integrity and reintegrates with minimal inflammation and post-surgical complications. The parent grant will pursue specific aims to evaluate the effectiveness of dura graft substitutes for dura graft replacement. Here we propose supplemental aims to investigate the role of astrocytes in healing following dura graft reintegration. The experiments and goals of the supplemental project will be incorporated into the parent grant’s methods to advance our understanding of the necessary qualities of dura graft substitutes for effective reintegration. The overall goal is for Ms. Ta-Tyonna Buck to investigate the role of astrocytes and the contributions they have to neural healing. In Supplemental Aim 1, Ta-Tyonna will utilize an in vitro model to assess the molecular mechanisms of astrocytes on the dura graft substitutes via a 3D co-culture system. In Supplemental Aim 2, she will assess the role of astrocytes in an in vivo model to measure the impact and reactivity of astrocytes following dura graft reintegration. Ta-Tyonna will gain an extensive skillset in biomedical imaging and microscopy, all of which are needed to progress in the proposed goals. Ta-Tyonna will meet weekly with Dr. Williams and the Williams lab for mentoring, research updates, feedback, and reviewing updated literature. Additionally, Ta-Tyonna will meet monthly with the parent grant investigator team for mentoring and updates on all studies. She will participate in workshops for research and writing success at UF, attend national conferences to present her work, and mentor undergraduates conducting research in the Williams Lab. Furthermore, she will attend professional development activities at conferences and research symposia to take advantage of their many networking and learning opportunities. The combined research and learning activities have been strategically outlined to support Ta-Tyonna’s progress throughout her Ph.D. and as she pursues a career in research.

项目摘要：颅脑硬脑膜在侵入性脑部手术期间被破坏，导致如果不及时治疗商业硬脑膜移植物可能致命的主要术后并发症。表现欠佳，因此迫切需要一种硬脑膜移植替代物来维持结构完整性并以最小的炎症和术后重新整合家长补助金将追求特定目标以评估其有效性。硬脑膜移植替代品在这里我们提出补充目标。研究星形胶质细胞在硬脑膜移植物重新整合后愈合中的作用。补充项目的目标将纳入父级资助的方法中，以我们对硬脑膜移植替代品必要品质的理解不断提高，以实现有效的重新融入社会。 Ta-Tyonna Buck 女士的总体目标是研究星形胶质细胞和在补充目标 1 中，Ta-Tyonna 将利用他们对神经治疗的贡献。评估硬脑膜移植物上星形胶质细胞分子机制的体外模型在补充目标 2 中，她将评估通过 3D 共培养系统替代的角色。体内模型中的星形胶质细胞用于测量硬脑膜后星形胶质细胞的影响和反应性移植物重新整合。 Ta-Tyonna 将获得生物医学成像和显微镜方面的广泛技能，所有这些都是 Ta-Tyonna 将每周与 Williams 博士会面并进行讨论。威廉姆斯实验室提供指导、研究更新、反馈和审查更新此外，Ta-Tyonna 每月都会与家长资助调查小组会面，以了解相关信息。她将参加研究和写作研讨会并提供所有研究的指导和更新。在佛罗里达大学取得成功，参加全国会议展示她的作品，并指导本科生此外，她还将在威廉姆斯实验室进行研究。会议和研究研讨会上的活动，以利用他们的众多网络和学习机会。战略性地概述了支持 Ta-Tyonna 在攻读博士学位期间的进步。研究事业。