Support of the Emory National Primate Research Center

埃默里国家灵长类动物研究中心的支持

基本信息

批准号：
10844283
负责人：
RAVI THADHANI
金额：
$ 16.99万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-08-01 至 2026-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10844283
关键词：
AIDS prevention Address Affinity Anatomy Antibodies Antibody Response Antigens B-Lymphocytes Binding Biopsy Bone Marrow CD4 Positive T Lymphocytes CD8-Positive T-Lymphocytes Cells Clinical Trials Conduct Clinical Trials Epidemic Follow-Up Studies Goals Grant HIV HIV vaccine HIV-1 HIV/AIDS Human Immune response Immunization Immunohistochemistry Infection prevention Inflammatory Intramuscular Knock-in Mouse Length Macaca mulatta Measures Messenger RNA Modified Vaccinia Virus Ankara Mucous Membrane Mutate Mutation Outcome Positioning Attribute Prevention Primates Production Proteins RNA vaccination Research Route SIV Sampling Scientist Structure of germinal center of lymph node T cell response T-Lymphocyte United States National Institutes of Health Vaccination Vaccines Viral Visualization coronavirus disease design experimental study gp160 immunogenicity improved mouse model neutralizing antibody nonhuman primate novel vaccines response subcutaneous tissue resident memory T cell vaccination strategy vaccine delivery vaccine development vaccine formulation vaccine strategy

项目摘要

Abstract The overall goal of this supplement is to develop vaccine strategies that induce strong neutralizing antibody response against HIV using mRNA and modified vaccinia Ankara (MVA) vaccine delivery platforms. To address the specific goals of the supplement, we will utilize samples obtained from studies conducted in the Emory Center for HIV/AIDS Vaccine and Cure Research consortium at the Emory National Primate Research Center. We propose to use the BG505 MD39 SOSIP mRNA as our vaccine strategy to elicit bnAbs. We hypothesize that BG505 MD39 mRNA delivered intramuscularly as either full length gp160 or truncated gp140 will induce HIV specific neutralizing antibodies with broad neutralization activity in rhesus macaques. Additionally, we would like to explore the effect of heterologous SIV Gag/Pol mRNA prime and MVA boost on the CD8 T cell response and evaluate how the route of immunization alters the ability of CD8 T cells to be boosted by MVA. We propose two specific aims. In Aim 1, we will compare the immunogenicity of MD39 gp160 and gp140 mRNA IM immunization. In Aim 2, we will characterize the immune response induced through heterologous mRNA prime and MVA boost and determine if the route of immunization effects the CD8 T cell response elicited by MVA. By completion of these aims we hope to develop improved vaccination strategy against HIV that can generate both neutralizing antibodies and T cells.

抽象的该补充剂的总体目标是开发诱导强中和抗体的疫苗策略使用 mRNA 和改良的安卡拉牛痘 (MVA) 疫苗递送平台来应对 HIV。到为了解决补充的具体目标，我们将利用从在埃默里国家灵长类动物研究中心艾滋病毒/艾滋病疫苗和治疗研究联盟中心。我们建议使用 BG505 MD39 SOSIP mRNA 作为我们的疫苗策略来引发 bnAb。我们假设 BG505 MD39 mRNA 作为全长 gp160 或截短的 gp140 肌肉内递送将在恒河猴中诱导具有广泛中和活性的 HIV 特异性中和抗体。此外，我们还想探讨异源 SIV Gag/Pol mRNA prime 和 MVA boost 对 CD8 T 细胞反应并评估免疫途径如何改变 CD8 T 细胞的能力受 MVA 推动。我们提出两个具体目标。在目标 1 中，我们将比较 MD39 gp160 的免疫原性和gp140 mRNA IM免疫。在目标 2 中，我们将描述通过以下方式诱导的免疫反应：异源 mRNA 引发和 MVA 增强并确定免疫途径是否影响 CD8 T 细胞 MVA 引起的响应。通过完成这些目标，我们希望制定改进的疫苗接种策略对抗 HIV，可以产生中和抗体和 T 细胞。

项目成果

期刊论文数量（760）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Image-Guided Transarterial Directed Delivery of Human Mesenchymal Stem Cells for Targeted Gastrointestinal Therapies in a Swine Model.

图像引导经动脉定向输送人类间充质干细胞，用于猪模型中的靶向胃肠道治疗。

DOI：
10.1016/j.jvir.2018.09.034
发表时间：
2019-03-06
期刊：
Journal of vascular and interventional radiology : JVIR
影响因子：
0
作者：
A. Prasanphanich;Christopher Johnson;Andrey Krasnopeyev;Shraddha I Cantara;Cristin Roach;S. Gumber;R. Chinnadurai;J. Galipeau;L. Brewster;J. Prologo
通讯作者：
J. Prologo

Genome-wide association study of positive emotion identifies a genetic variant and a role for microRNAs.

积极情绪的全基因组关联研究确定了 microRNA 的遗传变异和作用。

DOI：
发表时间：
2017-05
期刊：
影响因子：
11
作者：
Wingo, A P;Almli, L M;Stevens, J S;Jovanovic, T;Wingo, T S;Tharp, G;Li, Y;Lori, A;Briscione, M;Jin, P;Binder, E B;Bradley, B;Gibson, G;Ressler, K J
通讯作者：
Ressler, K J

Acute targeting of pre-amyloid seeds in transgenic mice reduces Alzheimer-like pathology later in life.

在转基因小鼠中急性靶向前淀粉样蛋白种子可减少晚年的阿尔茨海默样病理。

DOI：
发表时间：
2020
期刊：
影响因子：
25
作者：
Uhlmann, Ruth E;Rother, Christine;Rasmussen, Jay;Schelle, Juliane;Bergmann, Carina;Ullrich Gavilanes, Emily M;Fritschi, Sarah K;Buehler, Anika;Baumann, Frank;Skodras, Angelos;Al;Beschorner, Natalie;Ye, Lan;Kaeser, Stephan A;Obe
通讯作者：
Obe

A comprehensive atlas of white matter tracts in the chimpanzee.

黑猩猩白质束的综合图谱。