Genomics & Molecular Engineering Core

基因组学

• 批准号: 8011827
• 负责人: Nathan A. Ellis
• 金额: $ 19.93万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8011827
• 关键词: Advisory Committees; African American; Architecture; Asthma; Bacterial Artificial Chromosomes; Basic Science; Biochemistry; Biological Models; Cells; Charge; Chicago; Chromosomes, Human, Pair 3; Clinical; Clinical Trials; Colorectal Cancer; Committee Membership; Communication; Complex; Consultations; Core Facility; Cost Savings; Crohn' s disease; DNA; DNA Sequence; DNA Sequence Analysis; Data; Databases; Development; Digestive System Disorders; Disease; Disease Outcome; Ectopic Expression; Engineered Gene; Engineering; Ensure; Explosion; Faculty; Fee-for-Service Plans; Fostering; Future; Gastrointestinal tract structure; Gene Expression; Gene Silencing; Genes; Genetic; Genetic Engineering; Genetic Materials; Genetic Models; Genetic Polymorphism; Genetic Research; Genetic Services; Genetic Transcription; Genetic Variation; Genomics; Genotype; Grant; Human; Human Genome; Ice; Image; Image Analysis; Immunology; Individual; Inflammatory Bowel Diseases; Institution; Interdisciplinary Study; Intestinal Diseases; Knock-in Mouse; Knock-out; Knowledge; Leadership; Lentivirus Vector; Link; Mammalian Cell; Methodology; Methods; Microbe; Microbiology; Mission; Modality; Modems; Molecular; Molecular Biology; Mucositis; Mus; Mutation; Needs Assessment; Other Genetics; Outcome; Pathway interactions; Patients; Phenotype; Play; Population; Population Study; Preparation; Proteins; Proteomics; Reagent; Research; Research Personnel; Resource Sharing; Resources; Rheumatoid Arthritis; Risk; Risk Factors; Role; Rotation; Sampling; Scientific Advances and Accomplishments; Services; Severities; Single Nucleotide Polymorphism; Somatic Cell; Somatic Cell Genetics; Staging; Strategic Planning; System; Technology; Time; Tissues; Training; Translational Research; Ulcerative Colitis; Universities; Viral; base; cancer risk; cost; discount; empowered; functional genomics; gene function; genetic analysis; genetic manipulation; genetic technology; genome wide association study; homologous recombination; interest; meetings; member; new technology; novel; operation; protein expression; research study; treatment response

The Genomic and Molecular Engineering (GME) Core of the DDRCC provides cutting-edge technologies for the analysis and manipulation of the genetic material. These new technologies empower DDRCC members to pinpoint and characterize genetic factors that influence the development and course of digestive diseases and to dissect gene functions In important pathways relevant to digestive diseases. The GME is a new Core, restructured from the former Molecular Biology and Biochemistry Core. The GME Core divided into two components. The Genotype Analysis component offers sen/ices relating to the genetic analysis of patient samples. The services of the Genotype Analysis component Include (1) customized single nucleotide polymorphism (SNP) genotyping based on the Sequenom Massanray genotyping platfonn, (2) standard SNP genotyping panels for high-Interest genes, (3) ultra-high throughput DNA sequence analysis for genotyping, (4) other genotype analysis methods (such as TaqMan), (5) DNA preparation, and (6) statistical genetics support. The Genetic Engineering component offers services relating to the manipulation of genes in cellular and organismal model systems. The services ofthe Genetic Engineering component include (1) somatic cell genetic manipulation of genes using homologous recombination to knock in or knock out mutations, (2) recombineering technologies to manipulate large DNA segments in bacterial artificial chromosomes, (3) the construction of gene expression constructs using lentiviral vectors for ectopic expression or silencing of genes, (4) support for realtime PCR, and (5) support for the Odyssey image analysis system. The GME Core supports members for genotype analysis and genetic engineering experiments by supporting labor cost, providing discounts for reagents, and training members in new technologies. The Administrative Directors of the GME Core, Drs. Nathan Ellis and David Boone, oversee the operations of the respective components. Directors are responsible for ensuring proper scientific direction and efficient use of services and facilities of the component resources. Usage of the GME Core rapidly increased from start-up because of substantial cost savings, relevance, and high quality of services and resources, and it is anticipated usage will grow substantially during the next cycle of this Grant. Each of the Components offers training of new and established investigators unfamiliar with the supported experimental approaches. The GME Core has helped to foster multidisciplinary collaborations and promote productive exchanges brought about by sharing of resources

DDRCC的基因组和分子工程（GME）核心为分析和操纵遗传物质提供了尖端技术。这些新技术使DDRCC成员能够查明和表征影响消化疾病发展和过程的遗传因素，并在与消化系统疾病有关的重要途径中剖析基因功能。 GME是一个新的核心，由以前的分子生物学和生物化学核心进行重组。 GME核心分为两个组件。基因型分析成分提供了与患者样品的遗传分析有关的SEN/ICE。 The services of the Genotype Analysis component Include (1) customized single nucleotide polymorphism (SNP) genotyping based on the Sequenom Massanray genotyping platfonn, (2) standard SNP genotyping panels for high-Interest genes, (3) ultra-high throughput DNA sequence analysis for genotyping, (4) other genotype analysis methods (such as TaqMan), (5) DNA preparation, （6）统计遗传学支持。 基因工程组件提供了与细胞和有机模型系统中基因操纵有关的服务。 The services ofthe Genetic Engineering component include (1) somatic cell genetic manipulation of genes using homologous recombination to knock in or knock out mutations, (2) recombineering technologies to manipulate large DNA segments in bacterial artificial chromosomes, (3) the construction of gene expression constructs using lentiviral vectors for ectopic expression or silencing of genes, (4) support for realtime PCR, and （5）支持奥德赛图像分析系统。 GME核心通过支持人工成本，为试剂提供折扣以及新技术的培训成员来支持成员进行基因型分析和基因工程实验。 GME核心的行政总监Drs。内森·埃利斯（Nathan Ellis）和大卫·布恩（David Boone）监督各个组件的运营。董事负责确保零件资源的服务和设施的适当科学方向和有效利用。 GME核心的使用量从启动迅速增加，这是因为节省了大量成本，相关性以及高质量的服务和资源，并且预计在该赠款的下一个周期中，用法将大大增长。每个组件都提供对不熟悉所支持的实验方法的新的和既定的研究人员的培训。 GME Core帮助促进了多学科合作，并通过共享资源来促进产生的富有成效的交流