Structural Biology Core Facility

结构生物学核心设施

• 批准号: 8340313
• 负责人: Matthew R Redinbo
• 金额: $ 22.08万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-23 至 2015-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8340313
• 关键词: Accounting; Address; Antineoplastic Agents; Area; Basic Science; Bioinformatics; Biology; Biomolecular Nuclear Magnetic Resonance; Cancer Center; Cancer Center Support Grant; Chemicals; Complex; Computing Methodologies; Core Facility; Core Protein; Crystallization; Development; Drug Design; Drug Industry; Education; Environment; Equipment; Faculty; Funding; Grant; Homology Modeling; Housing; Human Resources; Lead; Malignant Neoplasms; Mechanics; Molecular Models; NMR Spectroscopy; Nucleic Acids; Peer Review; Pharmaceutical Preparations; Photons; Preparation; Process; Protein Dynamics; Proteins; Publications; Records; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Roentgen Rays; Sampling; Schedule; Scientist; Services; Source; Staging; Structural Biologist; Structure; Synchrotrons; Technology; X-Ray Crystallography; base; cost; design and construction; drug development; drug discovery; experience; inhibitor/antagonist; insight; macromolecule; member; molecular dynamics; molecular modeling; novel; protein complex; protein expression; protein purification; quantum; research study; structural biology; synergism; tool

Structural Biology Core Facility The Structural Biology Core is a 'super core' that provides an integrated platform of expertise, education, and infrastructure to make structural biology available as tools to LCCC researchers. The Core allows engaging in high-resolution studies using X-ray crystallography, multi-dimensional nuclear magnetic resonance spectroscopy and/or computational methods. The Core offers access to equipment for, and expert guidance of users through, all stages of structure determination projects: homology modeling, construct design, protein expression & purification, crystallization, structure determination, structure analysis, biophysical studies, molecular dynamics studies, presentation & publication. The utility of the available resources is demonstrated by numerous structural studies that contribute to the understanding of cancer-related processes at the atomic level and that can be used to develop potential new therapies through structure aided drug design. The Core is led by a team of highly experienced structural biologists with proven track records in cancer-related research In 2009, 32 LCCC members, all peer-reviewed accounted for 82% of total Core use.. With the recruitment of a director for the Core, Dr. Machius, in the summer of 2009, there has been a reorganization of the facilities, together with substantial renovations and equipment acquisition. As a result of the expanded services and increased demand, the number of projects is increasing sharply. Additional personnel are required to fulfill the needs of LCCC members. Renovations are currently underway to consolidate the Structural Biology facilities into contiguous space, providing a single point of access to resources and allowing for fighter integration of equipment and personnel. Also, an efficient interface is being formed between the Structural Biology Core and the Center for Integrative Chemical Biology and Drug Discovery (directed by Dr. Stephen Frye), which will establish a comprehensive pipeline available to LCCC members for the development of novel anti-cancer drugs based on insights gained from structural biology projects. For 2010, the LCCC requests $119,367, an increase of 29% for additional personnel). CCSG funds are projected to be 15% of operating costs.

结构生物学核心设施 结构生物学核心是一种“超级核心”，它提供了一个集成的专业知识，教育和基础设施平台，以使结构生物学作为LCCC研究人员的工具可用。核心允许使用X射线晶体学，多维核磁共振光谱和/或计算方法进行高分辨率研究。核心为设备提供访问和专家指导 通过用户通过结构确定的所有阶段：同源性建模，构造设计，蛋白质表达和纯化，结晶，结构确定，结构分析，生物物理研究，分子动力学研究，演示和出版。许多结构研究证明了可用资源的实用性，这有助于理解与癌症有关的理解 原子水平的过程，可用于通过结构辅助药物设计来开发潜在的新疗法。核心由一支经验丰富的结构生物学家团队领导 2009年，所有同行评审的32名LCCC成员占核心总使用量的82％。.随着核心董事Machius博士的招募，2009年夏天，设施进行了重组，并进行了大量的翻新和设备采购。由于服务的扩大和需求增加，项目数量正在急剧增加。需要其他人员来履行 LCCC成员的需求。 目前正在进行翻新，以将结构生物学设施巩固到连续的空间中，从而提供了单一的资源访问点，并允许设备和人员的战斗机整合。此外，在结构生物学核心与综合化学生物学和药物发现中心（由斯蒂芬·弗莱（Stephen Frye）指导）之间形成了有效的界面，该中心将建立一个 LCCC成员可根据结构生物学项目获得的见解来开发新型抗癌药物。对于2010年，LCCC要求$ 119,367，增加了29％的人员）。 CCSG资金预计将占运营成本的15％。