Slo2 channels in Olfactory Adaptation

嗅觉适应中的 Slo2 通道

• 批准号: 10823705
• 负责人: SUSAN L TSUNODA
• 金额: $ 41.21万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10823705
• 关键词: Action Potentials; Adult; Affect; Afferent Neurons; Behavioral; Biological Models; Brain; Cations; Cells; Code; Down-Regulation; Drosophila genus; Electrophysiology (science); Genes; Hyperactivity; Image; Ions; Microscopy; Modeling; Nature; Neurons; Olfactory Receptor Neurons; Organ; Orthologous Gene; Output; Play; Potassium Channel; Proteins; Receptor Signaling; Role; Seizures; Sensory; Signal Transduction; Site; Study models; Synapses; System; Testing; biophysical properties; desensitization; design; genetic manipulation; in vivo; insight; knock-down; neuronal excitability; patch clamp; receptor; response; sensory system

Project Summary Sensory adaptation is an important feature of all sensory systems, as it allows them to maintain sensitivity over a wide range in backgrounds of varying stimulation. Here, we introduce Na+-activated K+ (KNa/Slo2) channels a new player to the mechanisms that underlie sensory adaptation. Studies over the years have suggested that KNa/Slo2 channels might function to dampen activity in states of excessive excitation. KNa/Slo2 channels are unique in that the major depolarizing ion, Na+, activates a repolarizing K+-selective pore; thus by nature, these channels seem designed to be a built-in hyperpolarizing mechanism against episodes of excessive excitation. In the proposed studies, we use Drosophila as a model system in which to genetically manipulate dSlo2 expression, test for olfactory adaptation in vivo, identify the cells and subcellular domains in which dSlo2 channels affect adaptation, and electrophysiologically examine the role of dSlo2 channels in the excitability of these cells.

项目概要 感觉适应是所有感觉系统的一个重要特征，因为它使它们能够保持敏感性 在不同刺激的背景下范围广泛。在这里，我们介绍 Na+ 激活的 K+ (KNa/Slo2) 引导新玩家了解感官适应的机制。多年来的研究已 表明 KNa/Slo2 通道可能起到抑制过度兴奋状态下活动的作用。克钠/Slo2 通道的独特之处在于主要去极化离子 Na+ 激活复极化 K+ 选择性孔；因此通过 本质上，这些通道似乎被设计成一种内置的超极化机制，以防止过度的事件发生 励磁。在拟议的研究中，我们使用果蝇作为模型系统来进行遗传操纵 dSlo2 表达，测试体内嗅觉适应，识别 dSlo2 所在的细胞和亚细胞结构域 通道影响适应，并通过电生理学检查 dSlo2 通道在神经元兴奋性中的作用 这些细胞。