Recombinant production of structured fats that mimic human milk fat

重组生产模仿人乳脂肪的结构脂肪

基本信息

批准号：
10822765
负责人：
Aravind Somanchi
金额：
$ 37.06万
依托单位：
YALI BIOSCIENCES INC
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-25 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10822765
关键词：
Acyltransferase Address Biomanufacturing Bone Development Calcium Catalysis Cells Coconut Oil Codon Nucleotides DNA cassette Development Digestion Engineering Enzymes Fat Substitutes Fatty Acids Fatty acid glycerol esters Fermentation Food Formulation Foundations Future Genes Genomics Goals Growth Human Human Milk Hydration status Impairment In Vitro Infant Infant formula Lead Length Libraries Lipase Lipids Magnesium Marketing Mass Fragmentography Measures Methods Milk Newborn Infant Nonesterified Fatty Acids Nutritional Oils Oleates Organism Palmitates Pancreas Phase Phospholipids Plants Positioning Attribute Price Process Production Productivity Property Proteins Reaction Recombinants Repression Small Business Innovation Research Grant Soaps Source Specificity Stereoisomer Structure Sunflower Oil System Technology Transgenic Organisms Triglycerides Vegetable Oils Weight Work Yarrowia Yarrowia lipolytica Yeasts absorption alpha-glycerophosphoric acid cost cost effective diacylglycerol O-acyltransferase dietary requirement infant nutrition innovation liquid chromatography mass spectrometry manufacture metabolic engineering milk fat nutrient absorption nutrition overexpression palm oil scale up success supply chain technology platform unsaturated bonds

项目摘要

Abstract Human breast milk is the choice nutrition for infants, and the fat component of the milk (human milk fat, HMF) supplies the highest fraction of the infant's required dietary energy. The fats within human breast milk are triacylglycerols (TAGs) with a unique stereoisomeric structure that facilitates efficient nutrient absorption in the infant gut. In contrast, vegetable oils form the main source of fats in commercial infant formulas: the composition and distribution of the fatty acids in vegetable oils are different from those in human milk which hinders nutrient absorption in infants, and consequently their growth. To mimic the nutritional functions of human milk fat, human milk fat substitutes (HMFS) have been developed through enzymatic interesterification reactions in recent years. Human milk fat substitutes (HMFS) produced by in vitro enzyme-catalysis of purified vegetable oils, have the desired properties but are challenging to manufacture with high cost. Yali Bio aims to use its recombinant yeast platform to provide a biomanufacturing solution for high quality HMFS that is cost-effective. We propose to use our platform to efficiently produce structured fats that resemble human milk fat in their physicochemical properties and generate highly productive, proprietary yeast strains accumulating fats with a stereoisomeric composition similar to human milk fats. The approach described here should lead to a cost-effective biomanufacturing method for recombinant production of structured fats that mimic human milk fats.

抽象的母乳是婴儿的首选营养品，乳汁中的脂肪成分（母乳）脂肪（HMF）提供了婴儿所需膳食能量的最高部分。人体内的脂肪母乳是三酰甘油（TAG），具有独特的立体异构结构，有助于高效婴儿肠道的营养吸收。相比之下，植物油是人体脂肪的主要来源。商业婴儿配方奶粉：植物油中脂肪酸的组成和分布是与母乳中的不同，会阻碍婴儿的营养吸收，因此它们生长。为了模仿母乳脂肪的营养功能，母乳脂肪替代品 (HMFS) 近年来通过酶促酯交换反应发展起来。人乳脂肪通过纯化植物油的体外酶催化产生的替代品（HMFS），具有所需的但其制造成本较高，具有挑战性。雅利生物旨在利用其重组酵母平台，为高品质 HMFS 提供经济高效的生物制造解决方案。我们建议使用我们的平台高效生产结构脂肪，其结构类似于人乳脂肪理化特性并产生高产的、积累脂肪的专有酵母菌株具有与人乳脂肪相似的立体异构组成。这里描述的方法应该导致一种具有成本效益的生物制造方法，用于重组生产结构脂肪，模仿人乳脂肪。