Advanced optics to unlock large field-of-view multiphoton imaging for neuroscience and beyond

先进的光学器件可为神经科学及其他领域解锁大视场多光子成像

• 批准号: 10829782
• 负责人: SPENCER LAVERE SMITH
• 金额: $ 29.26万
• 依托单位: PACIFICOPTICA LLC
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-19 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10829782
• 关键词: Address; Adoption; Air; Animals; Area; Avocado; BRAIN initiative; Biology; Businesses; Calcium; Cells; Country; Creativeness; Custom; Data; Dendrites; Development; Diameter; Docking; Frequencies; Funding; Goals; Health; Image; Immersion; Knowledge; Laboratories; Marketing; Measures; Microscope; Neurobiology; Neurons; Neurosciences; Optics; Performance; Reporting; Research Design; Resolution; Scanning; Speed; Standardization; Synapses; System; Technical Expertise; Technology; Technology Transfer; Testing; Universities; Vision; Work; biomedical imaging; commercialization; design; experimental study; flexibility; high resolution imaging; imaging system; improved; innovation; lens; multi-photon; multiphoton imaging; multiphoton microscopy; neuroimaging; novel; novel strategies; open source; prototype; two-photon; usability

Project summary Currently available optical components limit biomedical imaging. In particular, in neurobiology, standard microscope components limit the field-of-view and speed of imaging of neuronal activity. Through prior work on BRAIN Initiative project, we have developed approaches for new optical components that expand the field-of-view 5 to 50 times over standard microscopes, with no loss in resolution, while retaining high imaging speed. In this project, we will develop three new products to further impact this field, enabling better imaging systems, higher quality data, and new experiments. The three products are: (Aim 1) a new long working distance air objective with high resolution, (Aim 2) a high performance scan lens for large field-of-view imaging, and (Aim 3) an angle-doubling module for large field-of-view imaging with no loss in scan speed. The company will perform the design studies and development. The company will partner with the university lab to validate and evaluate the products in state-of-the-art experiments. The results from this project will result in innovative new optical components that will expand the field-of-view and throughput of imaging systems in neurobiology labs.

项目概要 目前可用的光学元件限制了生物医学成像。特别是在神经生物学中，标准显微镜 组件限制了神经元活动成像的视野和速度。通过之前对 BRAIN 的研究 在倡议项目中，我们开发了新光学元件的方法，可将视场扩大 5 至 50 是标准显微镜的两倍，分辨率没有损失，同时保持高成像速度。在这个项目中， 我们将开发三款新产品来进一步影响这一领域，实现更好的成像系统、更高的质量 数据和新实验。这三款产品是：（目标1）新型长工作距离空气物镜，具有高 分辨率，（目标 2）用于大视场成像的高性能扫描镜头，以及（目标 3）倍角 用于大视场成像的模块，且不会降低扫描速度。公司将进行设计研究 和发展。该公司将与大学实验室合作验证和评估产品 最先进的实验。该项目的成果将产生创新的新型光学元件 将扩大神经生物学实验室成像系统的视野和吞吐量。