Spine Dynamics Underlying Ocular Dominance Plasticity

眼部优势可塑性背后的脊柱动力学

基本信息

批准号：
7047748
负责人：
SPENCER LAVERE SMITH
金额：
$ 3.62万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-01 至 2006-12-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): In the developing nervous system, initially promiscuous synaptic connections are remodeled by activity-dependent mechanisms, with axons strengthening their connections with some targets while completely disconnecting from others. In the neocortex, this process has been most thoroughly studied in the developing primary visual cortex, where decorrelating the activity of the two eyes dramatically reduces cortical binocularity. These functional changes in cortical physiology are widely accepted to be anchored in long-lasting changes in the efficacy of synaptic connections. However, the specific mechanisms by which synaptic plasticities lead to changes in the function of cortical circuitry remain elusive. The major objective of the work proposed here is to determine where in the cortical circuit experience-dependent changes in binocular responses are first observed, track the progression over time, and test the hypothesis that these changes are anchored in anatomical changes in synaptic connectivity. To address these questions, techniques such as 2-photon laser scanning microscopy, intrinsic signal optical imaging, and single-unit recordings, will be used to map circuit plasticity and follow changes in synaptic connectivity in vivo.

描述（由申请人提供）：在发育中的神经系统中，最初混杂的突触连接通过活动依赖性机制进行重塑，轴突加强了与某些目标的连接，同时与其他目标完全断开。在新皮层中，这一过程在发育中的初级视觉皮层中得到了最彻底的研究，在该皮层中，两只眼睛的活动去相关会显着降低皮层的双眼视度。人们普遍认为，皮质生理学的这些功能变化与突触连接功效的长期变化有关。然而，突触可塑性导致皮质电路功能变化的具体机制仍然难以捉摸。这里提出的工作的主要目标是确定在皮质回路中首先观察到双眼反应的经验依赖性变化的位置，跟踪随时间的进展，并测试这些变化锚定于突触连接的解剖学变化的假设。为了解决这些问题，将使用 2 光子激光扫描显微镜、固有信号光学成像和单单元记录等技术来绘制电路可塑性并跟踪体内突触连接的变化。