The National Center for In-situ Tomographic Ultramicroscopy (NCITU)

国家原位断层超显微术中心 (NCITU)

• 批准号: 10818768
• 负责人: Jeffrey S Kieft
• 金额: $ 35万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10818768
• 关键词: Cells; Cryo-electron tomography; Electron Microscopy; Equipment; Funding; In Situ; New York; Preparation; Qualifying; Sampling; Services; Specimen; Time; Training; Visualization; Work; computing resources; equipment acquisition; instrument; light microscopy; method development; structural biology; tomography

Project Summary/Abstract The National Center for In-situ Tomographic Ultramicroscopy (NCITU), based at the New York Structural Biology Center, provides user access and service towards preparing specimens for direct visualization of macromolecular associations and organization, particularly in native cells. A highly qualified technical team provides direct hands-on service, guidance, and assistance to ensure that samples and grids are prepared with the highest quality and in the shortest time possible. We are requesting funds to purchase equipment to upgrade and expand our computational resources and upgrade our Thermo Fisher Scientific (TFS) Aquilos2 for correlative light and electron microscopy (CLEM) work. These upgrades will benefit all users of NCITU by increasing the capability and capacity of these instruments.

项目概要/摘要 国家原位断层超显微术中心 (NCITU)，位于纽约 结构生物学中心，为用户提供准备样本的访问和服务 大分子关联和组织的直接可视化，特别是在天然细胞中。 高素质的技术团队为您提供直接的实践服务、指导和帮助 确保在最短的时间内以最高的质量制备样品和网格 可能的。 我们正在请求资金购买设备以升级和扩展我们的计算 资源并升级我们的 Thermo Fisher Scientific (TFS) Aquilos2，以获得相关光和 电子显微镜（CLEM）工作。这些升级将使 NCITU 的所有用户受益，增加 这些仪器的能力和能力。

项目成果

In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method.

使用华夫饼法进行原位冷冻 FIB/SEM 样品制备。

• 发表时间: 2022-11-05
• 影响因子: 0.8
• 作者: Klykov, Oleg;Bobe, Daija;Paraan, Mohammadreza;Johnston, Jake D;Potter, Clinton S;Carragher, Bridget;Kopylov, Mykhailo;Noble, Alex J
• 通讯作者: Noble, Alex J