Biophysics Studies of Protein, Nucleic Acids, and Viruses

蛋白质、核酸和病毒的生物物理学研究

• 批准号: 8294617
• 负责人: Cynthia Vianne Stauffacher
• 金额: $ 11.12万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8294617
• 关键词: Biophysics; Nucleic Acids; Proteins; Virus

DESCRIPTION (provided by applicant): This Molecular Biophysics pre-doctoral training program involves sixteen mentors from four degree-granting departments and a Purdue-wide life sciences interdisciplinary program, who provide research training linked to studies of the structure and function of proteins, nuclei acids and viruses. The program emphasizes training in three major structural subdisciplines, X-ray crystallography, electron microscopy and image analysis and multidimensional NMR spectroscopy, with a newly added emphasis on protein bioinformatics. Since biophysics and structural biology are not traditional undergraduate disciplines, the curriculum includes courses that offer entry points for students from diverse backgrounds with differing knowledge of biology, chemistry, physics and mathematics, as well as advanced courses that add deeper theoretical and practical understanding. The Program also delivers significant extracurricular experiences that insure breadth of knowledge and prepare the trainees for careers as scientists. Among these are an annual student-organized and student-presented Biophysics Symposium and a series of mentor-organized Biophysical Discussions that focus on the scientific and technical context of key seminars. The faculty mentors have a long and successful history in collaborative research and training that enriches the program and benefits the trainees. Superb major research facilities for crystallography, NMR and electron microscopy have been established and maintained as shared facilities available to all trainees. New facilities to house laboratories for trainees and their mentors are now being constructed, with the entire three floors of the new Hockmeyer Hall building dedicated to structural biophysics research.

描述（由申请人提供）：该分子生物物理学博士前培训计划涉及来自四个授予部门的16位导师和一项范围内的Purdue Life Sciences跨学科计划，他们提供了与蛋白质，核酸酸和病毒的结构和功能有关的研究培训。该计划强调了三个主要的结构子学科，X射线晶体学，电子显微镜和图像分析以及多维NMR光谱的训练，并新添加了对蛋白质生物信息学的重点。由于生物物理学和结构生物学不是传统的本科学科，因此该课程包括为来自不同背景的学生提供对生物学，化学，物理学和数学知识不同的学生提供的入口，以及增加了更深入的理论和实践理解的高级课程。该计划还提供了重要的课外经验，可确保知识广泛，并为受训者做好作为科学家的职业做好准备。其中包括一年一度的学生组织和学生提供的生物物理学研讨会，以及一系列由导师组织的生物物理讨论，重点是关键研讨会的科学和技术背景。教师导师在合作研究和培训方面拥有悠久而成功的历史，可以丰富计划并使受训者受益。已经建立并维护了所有学员可用的共享设施，用于晶体学，NMR和电子显微镜的精湛主要研究设施。现在正在建造为学员及其导师提供实验室的新设施，而新的Hockmeyer Hall大楼的整个三层楼都专门用于结构生物物理学研究。

项目成果

Probing the early temporal and spatial interaction of the Sindbis virus capsid and E2 proteins with reverse genetics.

通过反向遗传学探讨辛德比斯病毒衣壳和 E2 蛋白的早期时空相互作用。

• DOI: 10.1128/jvi.01220-12
• 发表时间: 2012
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Snyder,JonathanE;Berrios,ChristianJ;Edwards,ThomasJ;Jose,Joyce;Perera,Rushika;Kuhn,RichardJ
• 通讯作者: Kuhn,RichardJ

Identification and comparative analysis of hepatitis C virus-host cell protein interactions.

• DOI: 10.1039/c3mb70343f
• 发表时间: 2013-12
• 期刊: Molecular bioSystems
• 作者: Dolan PT;Zhang C;Khadka S;Arumugaswami V;Vangeloff AD;Heaton NS;Sahasrabudhe S;Randall G;Sun R;LaCount DJ
• 通讯作者: LaCount DJ

Crystallographic analysis of the ENTH domain from yeast epsin Ent2 that induces a cell division phenotype.

对诱导细胞分裂表型的酵母 epsin Ent2 的 ENTH 结构域进行晶体学分析。

• DOI: 10.1002/pro.2259
• 发表时间: 2013
• 期刊: Protein science : a publication of the Protein Society
• 作者: Costakes,GregoryT;Sen,Arpita;Aguilar,RClaudio;Stauffacher,CynthiaV
• 通讯作者: Stauffacher,CynthiaV

Rescue of infectious particles from preassembled alphavirus nucleocapsid cores.

从预组装的甲病毒核衣壳核心中拯救感染性颗粒。

• DOI: 10.1128/jvi.00039-11
• 发表时间: 2011
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Snyder,JonathanE;Azizgolshani,Odisse;Wu,Bingbing;He,Yingpei;Lee,AihCheun;Jose,Joyce;Suter,DanielM;Knobler,CharlesM;Gelbart,WilliamM;Kuhn,RichardJ
• 通讯作者: Kuhn,RichardJ

A novel role for coenzyme A during hydride transfer in 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

辅酶 A 在 3-羟基-3-甲基戊二酰辅酶 A 还原酶的氢化物转移过程中的新作用。

• DOI: 10.1021/bi400335g
• 发表时间: 2013
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Steussy,CNicklaus;Critchelow,ChandraJ;Schmidt,Tim;Min,Jung-Ki;Wrensford,LouiseV;Burgner2nd,JohnW;Rodwell,VictorW;Stauffacher,CynthiaV
• 通讯作者: Stauffacher,CynthiaV