Nanosensor device for the detection of H. Pylori and prevention of gastric cancer

用于检测幽门螺杆菌和预防胃癌的纳米传感器装置

• 批准号: 8114329
• 负责人: Anastasia Rigas
• 金额: $ 15.23万
• 依托单位: NANOMEDICON, LLC
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8114329
• 关键词: Air; Ammonia; Antibiotics; Bacteria; Biological Assay; Breath Tests; Carbon Dioxide; Clinical; Clinical Management; Data; Detection; Developing Countries; Development; Devices; Diagnosis; Diagnostic tests; Disease; Emerging Technologies; Enzymes; Gastric Adenocarcinoma; Gastric lymphoma; Goals; Hand; Healthcare; Helicobacter Infections; Helicobacter pylori; Human; Human Resources; Infection; Label; Lymphoma; Medical; Methods; Monitor; Nanotechnology; Numerical value; Organism; Patients; Peptic Ulcer; Population; Price; Proteins; Public Health; Risk; Sampling; Sensitivity and Specificity; Testing; Treatment Efficacy; Urea; Urease; Work; base; cost; gastric cancer prevention; human disease; instrument; instrumentation; malignant stomach neoplasm; molybdenum trioxide; mucosa-associated lymphoid tissue lymphoma; nanosensors; novel; point of care; prevent; sensor

DESCRIPTION (provided by applicant): We propose to develop a novel method for the diagnosis of Helicobacter Pylori. The diagnosis of H. Pylori is a high medical priority because: this bacterium infects approximately 50% of the world's population; causes peptic ulcer disease, gastric cancer, a form of lymphoma (MALToma) and likely several other diseases; and is treatable with antibiotics, but if this organism is not eradicated, patients remain at risk for serious complications. Of the many diagnostic tests for H Pylori, the urea breath test (UBT) is the best: it is simple, noninvasive, has excellent sensitivity and specificity and can diagnose current infection. It is based on the ability of H pylori to convert urea to CO2 and ammonia using its enzyme urease. Current embodiments of UBT employ 13C-urea and detect labeled CO2 in breath. Current UBTs are not extensively used because they are costly and not available at the point-of-care. We propose to develop a simple UBT using as substrate unlabeled urea and detecting ammonia in breath with a nanosensor incorporated in a very simple device. Our preliminary work fully supports the feasibility of our approach. Our specific aims are to 1) Perform UBTs in patients using our sensor to determine NH3, and 2) Develop a portable breath analyzer for the detection of H. pylori based on the determination of NH3 in human breath. The proposed UBT will be simple, inexpensive, noninvasive and available at the point-of care. Thus it will make widely available a simple device for the diagnosis of H pylori and the monitoring of treatment efficacy. PUBLIC HEALTH RELEVANCE: We propose to develop a simple-to-use, inexpensive and point of care device to rapidly diagnose infection with Helicobacter pylori. This organism infects approximately 50% of the world's population and causes peptic ulcer. It has also been associated with the development of gastric cancer. The device's impact on public health will be immediate and major.

描述（由申请人提供）：我们建议开发一种诊断幽门螺杆菌的新方法。幽门螺杆菌的诊断是一个很高的医疗优先事项，因为：这种细菌感染了大约50％的世界人口；引起消化性溃疡病，胃癌，一种淋巴瘤（Maltoma）形式以及可能其他几种疾病。并且可以用抗生素治疗，但是如果未消除这种生物，患者仍有严重并发症的风险。在许多H幽门螺杆菌的诊断测试中，尿素呼气测试（UBT）是最好的：它是简单的，无创的，具有出色的灵敏度和特异性，并且可以诊断当前感染。它基于幽门螺杆菌使用其酶尿素酶将尿素转化为二氧化碳和氨的能力。 UBT的当前实施方案采用13C-rea并在呼吸中检测标记的CO2。当前的UBT并未广泛使用，因为它们在护理点上是昂贵且无法使用的。我们建议使用AS底物未标记的尿素来开发简单的UBT，并使用在非常简单的设备中掺入纳米传感器呼吸中的氨。我们的初步工作完全支持我们方法的可行性。我们的具体目的是1）使用传感器确定NH3的患者对患者进行UBT，以及2）基于人类呼吸中NH3的确定，开发一种便携式呼吸分析仪来检测幽门螺杆菌。拟议的UBT将是简单，廉价，无创的，并在医疗点上可用。因此，它将使广泛使用的简单设备用于诊断幽门螺杆菌和监测治疗功效。 公共卫生相关性：我们建议开发一种简单的，廉价和护理设备，以快速诊断幽门螺杆菌感染。这种生物体感染了世界大约50％的人口，并导致消化性溃疡。它也与胃癌的发展有关。该设备对公共卫生的影响将是直接和重大的。