Clinical and Microbiology Laboratory Core

临床和微生物学实验室核心

• 批准号: 8138520
• 负责人: MARCIA METZGAR HOBBS
• 金额: $ 32.13万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8138520
• 关键词: Authorization documentation; Bacterial Adhesins; Biological; Biological Markers; China; Clinic; Clinical; Clinical Microbiology; Clinical Research; Collaborations; Collection; County; Data; Detection; Development; Diagnostic; Diagnostic tests; Engineering; Epidemiology; Evaluation; Family health status; Funding; Future; Genotype; Gonorrhea; Human; Human Resources; Immune response; Infection; Institution; International; Iron; Jamaica; Knowledge; Laboratories; Madagascar; Malawi; Membrane Proteins; Neisseria gonorrhoeae; Pathogenesis; Peptidoglycan; Peru; Phase; Pilum; Prostate-Specific Antigen; Proteins; Proteomics; Protocols documentation; Public Health; Research; Research Ethics Committees; Research Infrastructure; Research Institute; Research Personnel; Research Project Grants; Resources; Role; Seminal fluid; Sexually Transmitted Diseases; Source; Specimen; Structure; System; Techniques; Technology; Testing; Trichomonas Infections; Trichomonas vaginalis; Universities; Urethra; Variant; Virginia; Washington; Wisconsin; Woman; antimicrobial; base; clinical care; design; efflux pump; human subject; lipooligosaccharide; male; men; microbial; novel diagnostics; pathogen; repository; research study; volunteer

The Clinical and Microbiology Laboratory Core (Micro Core B) supports 4 Projects in the Southeastern STI Cooperative Research Center and provides a broad base of support for other local, national and international STI research projects. We will conduct experimental human infection studies with Neisseria gonorrhoeae to determine the requirements for the gonococcal antimicrobial efflux pump, lipooligosaccharide structures and phase variation (Project 3, Shafer); pilus-associated proteins (Project 4, Thomas); iron-regulated adhesins (Project 2, Comelissen); and peptidoglycan release (Joseph Dillard, Univ. of Wisconsin) for urethral infection in men. In addition, clinical specimens from experimentally infected subjects will be collected for analysis to evaluate innate immune responses to experimental gonococcal infection (Project 5, Duncan). To facilitate the identification and characterization of iron-regulated surface proteins in N. gonorrhoeae that may function as adhesins, the Micro Core will coordinate interactions between Dr. Comelissen in Project 2 at the Virginia Commonwealth University and the UNC-Duke Michael Hooker Proteomics Center, located on the UNCChapel Hill campus. In addition, the Micro Core will serve as a repository of clinical and biological specimens from a variety of studies for future STI testing. We will continue to evaluate new diagnostic tests for sexually transmitted pathogens. In particular, we will evalute a T. vaginalis genotyping system (developed as a project in the previous NC STI-TM CRC) using recent specimens from HIV-infected women with trichomoniasis participating in an ongoing clinical study in the Gulf South STI CRC and T. vaginalis isolates from women in a recently completed study conducted by the UW STI CRC in Peru. Other activities will include the evaluation and use of tests for biomarkers of recent semen exposure, including detection of prostate-specific antigen. In addition, the Micro Core will continue to perform STI diagnostic testing in support of clinical research studies.

临床和微生物实验室核心（微核B）支持东南部的4个项目 合作研究中心，为其他本地，国家和国际提供广泛的支持基础 STI研究项目。我们将用淋病奈瑟氏菌进行实验性人类感染研究 确定淋球菌抗菌外排泵的要求，脂肪含糖结构和 相位变化（项目3，Shafer）；菌毛相关的蛋白质（项目4，托马斯）；铁调节的粘附素 （Project 2，Comelissen）；尿道感染的肽聚糖释放（威斯康星州的约瑟夫·迪拉德（Joseph Dillard），威斯康星州大学） 在男人。此外，将收集来自实验感染受试者的临床标本以进行分析 评估对实验性淋球菌感染的先天免疫反应（项目5，邓肯）。促进 在淋病猪笼草中的铁调节表面蛋白的鉴定和表征可能起作用 作为粘合剂，微心将在弗吉尼亚州项目2中的Comelissen博士之间进行协调 英联邦大学和UNC-Duke Michael Hooker蛋白质组学中心，位于UNCCHAPEL 希尔校园。此外，微芯将作为临床和生物学的存储库 来自各种研究的样本，用于未来的STI测试。我们将继续评估新的诊断测试 用于性传播病原体。特别是，我们将评估阴道猪笼草基因分型系统 （在先前的NC STI-TM CRC中作为项目开发）使用HIV感染妇女的最新标本 随着毛诺病参加在海湾South STI CRC和T. Vaginalis的一项正在进行的临床研究 UW STI CRC在秘鲁进行的一项最近完成的研究中，妇女的分离株。其他活动 将包括评估和使用最近精液暴露的生物标志物的测试，包括检测 前列腺特异性抗原。此外，微芯将继续执行STI诊断测试 临床研究的支持。