Trichomonas Typing System

毛滴虫分型系统

• 批准号: 6866279
• 负责人: MARCIA METZGAR HOBBS
• 金额: $ 19.98万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6866279
• 关键词: Trichomonas vaginalis; clinical research; cooperative study; female; genetic polymorphism; genetic strain; genotype; human subject; polymerase chain reaction; sex partner; sexually transmitted diseases; vagina

Trichomonas vaginalis (Tv) accounts for nearly half of all curable sexually transmitted infections worldwide, including 5 million new infections annually in the US. Though infection is often asymptomatic in men and women, urethritis, vaginitis and cervicitis are not uncommon. In women, Tv has been associated with adverse birth outcomes, and infection is an important co-factor in HIV transmission. The extent of Tv strain variation and whether some strains are more likely to produce symptomatic or asymptomatic infection are not known. Reports of resistance to metronidazole have appeared and are increasing, but there have been few studies documenting the true extent of treatment failures, since distinction between reinfection and persistent infection await a practical typing system. Development of a reliable, molecular Tv typing system would improve understanding the epidemiology of Tv infection, resulting in better detection and treatment of trichomoniasis, which in turn are needed improve women's reproductive health and reduce HIV transmission where Tv is common. The specific aims are 1) To establish Tv genotypes based on sequence and length polymorphisms at sites in the parasite genome containing tandem DNA repeats. Loci containing variable numbers of tandem repeats will be amplified by PCR. Allele length polymorphisms will be exploited to generate a multi-locus typing system. 2) To define the genetic heterogeneity of Tv viruses (TVV) in recent clinical isolates to identify subgroups of TVV+ strains. Viral polymerase cDNA sequences will be generated and compared to reveal the relatedness and clustering patterns of TVVs. 3) To examine strain types of existing clinical specimens including Tv from sexual partners. Using the typing system developed in Aims 1 and 2, we will compare T. vaginalis strain types from infected women and their male partners attending two STD clinics in NC and one in AL to define the extent of strain differences within and between sexual partnerships and within and between clinic populations. We will also test the hypotheses that differences in Tv strain types contribute to clinical presentation and transmission by comparing the distribution of Tv types among women with or without symptoms and whose partners were or were not infected with Tv. The results of these studies may lead to the development of methods to identify particularly important Tv strains, improving treatment, and thereby reducing the adverse health consequences of this enormously prevalent STI.

Vaginalis（TV）占全球所有可治愈的性传播感染的一半，其中包括每年500万种新感染。尽管男性和女性的感染通常是无症状的，但尿道炎，阴道炎和宫颈炎并不少见。在女性中，电视与不良的出生结果有关，感染是艾滋病毒传播中重要的副因素。电视菌株变化的程度以及某些菌株是否更有可能产生症状或无症状感染。关于甲硝唑抗性的报道已经出现并且正在增加，但是很少有研究记录了治疗失败的真实程度，因为再感染和持续感染之间的区别在等待实用的分类系统。开发可靠的，分子电视打字系统将改善理解电视感染的流行病学，从而更好地检测和治疗毛滴虫病，而这反过来又需要改善妇女 生殖健康并减少电视常见的艾滋病毒传播。具体目的是1）基于含有串联DNA重复序列的寄生虫基因组的位点的序列和长度多态性建立电视基因型。包含可变数量的串联重复序列的基因座将通过PCR扩增。等位基因长度多态性将被利用以生成多洛克斯打字系统。 2）在最近的临床分离株中定义电视病毒（TVV）的遗传异质性，以鉴定TVV+菌株的亚组。将生成病毒聚合酶cDNA序列并进行比较，以揭示TVV的相关性和聚类模式。 3）检查现有的临床标本的应变类型，包括性伴侣的电视。使用AIM 1和2中开发的打字系统，我们将比较受感染的妇女及其男性伴侣的阴道菌株类型，他们在NC的两个性病诊所，一个在AL中进行了一个，以确定性伙伴关系内部和内部和诊所种群之间的菌株差异的程度。我们还将测试电视应变差异的假设 通过比较有或没有症状的女性，其伴侣或未感染电视的女性中电视类型的分布，从而有助于临床表现和传播。这些研究的结果可能导致开发方法，以确定特别重要的电视菌株，改善治疗，从而减少这种极为普遍的STI的不良健康后果。