Circadian regulation of brain and body in larval zebrafish

斑马鱼幼体大脑和身体的昼夜节律调节

基本信息

批准号：
10798875
负责人：
DAVID Edward EHRLICH
金额：
$ 25万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10798875
关键词：
3-Dimensional Ablation Activity Cycles Affect Animal Behavior Animals Arousal Award Behavior Behavioral Biomechanics Brain Brain Stem Calcium Cells Chronobiology Circadian Rhythms Complement Complex Confocal Microscopy Darkness Disease Equipment Fishes Fluorescence Funding Goals Hormones Image Individual Institution Laboratories Lasers Lesion Light Lighting Measurement Measures Metabolism Methods Microscope Microscopy Modeling Muscle Nervous System Neurology Neuromodulator Neurons Neuropeptides Nuclear Optics Organ Output Pacemakers Phase Photons Photosensitizing Agents Physiological Physiology Population Postdoctoral Fellow Research Research Design Resolution Resources Services Signal Pathway Signal Transduction Signaling Molecule Slave Sleep disturbances Specificity Structure System Time Tissues Transgenic Organisms Visible Radiation Wakefulness Work Zebrafish calcium indicator cellular targeting circadian circadian regulation experience experimental study fluorophore genetic approach hypocretin instructor multi-photon multiphoton microscopy muscle physiology neural receptor sleep behavior time use vibration

项目摘要

Project Summary Circadian rhythms drastically alter animal behavior through diverse actions on cellular targets throughout the body. Disruptions of sleep-activity cycles account for a wide range of diseases affecting behavior, neurology, metabolism, and muscle physiology. This proposal presents a set of studies designed to understand the mechanisms and interactions of circadian effects on cells and systems spanning the body. Circadian rhythms manifest downstream of pacemakers via signaling molecules that act directly on targets to regulate physiology. Understanding how distinct targets are modified to achieve a constellation of physiological and behavioral rhythms is a major goal in chronobiological research. We study how neurons, muscles, and biomechanics interact in larval zebrafish, a tractable diurnal vertebrate, and our preliminary experiments suggest these animals experience a breadth of circadian changes far more diverse than previously known. Although the zebrafish is an imperfect model of sleep behavior, its clear circadian rhythms combined with its tractability for physiological, behavioral, and genetic approaches make it an ideal system for understanding how circadian rhythms organize and interact across cells and organs. The original proposal aims to define how diverse circadian effects on nervous system output and muscle physiology amount to complex behavioral output, by tracking and modeling zebrafish behavior and arousal across the diel cycle. Here we request resources to complement institutional support for a multiphoton microscope, which affords activity measurements using invisible light, thereby facilitating comparisons of physiology across circadian time without the confounding effects of measurement approaches that use visible light. Together these experiments will provide detailed information and models regarding the interaction of circadian effects across cells and systems.

项目概要昼夜节律通过对整个细胞目标的不同作用而极大地改变动物的行为身体。睡眠活动周期的破坏导致了一系列影响行为、神经病学、新陈代谢和肌肉生理学。该提案提出了一系列研究，旨在了解昼夜节律对全身细胞和系统影响的机制和相互作用。昼夜节律通过直接作用于目标来调节生理机能的信号分子表现在起搏器下游。了解如何修改不同的目标以实现一系列生理和行为节律是时间生物学研究的一个主要目标。我们研究神经元、肌肉和生物力学如何斑马鱼幼体是一种易驯化的昼间脊椎动物，我们的初步实验表明这些动物经历比以前已知的更加多样化的昼夜节律变化。虽然斑马鱼是一种睡眠行为的不完美模型，其清晰的昼夜节律与其生理的易处理性相结合，行为和遗传方法使其成为了解昼夜节律如何组织的理想系统并在细胞和器官之间相互作用。最初的提案旨在定义不同的昼夜节律如何影响通过跟踪和建模，神经系统输出和肌肉生理学相当于复杂的行为输出斑马鱼整个昼夜周期的行为和唤醒。在这里，我们请求资源来补充机构支持多光子显微镜，使用不可见光进行活动测量，从而促进跨昼夜节律时间的生理学比较，而不会产生测量的混淆效应使用可见光的方法。这些实验将共同提供详细的信息和模型关于跨细胞和系统的昼夜节律效应的相互作用。