Research Project 1 - Developing and applying tools to probe internal state dynamics of perception and motivation

研究项目 1 - 开发和应用工具来探测感知和动机的内部状态动态

基本信息

批准号：
10687144
负责人：
Karl A. Deisseroth
金额：
$ 83.03万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-17 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10687144
关键词：
3-Dimensional Animal Behavior Area Astronomy Behavior Behavioral Behavioral Model Biological Brain Brain region Calibration Cell Nucleus Cells Communities Complex Data Data Set Development Diameter Dimensions Doctor of Philosophy Education and Outreach Ensure Event Fire - disasters Gene Expression Individual Lead Learning Link Measures Methods Mission Modeling Molecular Motivation Mus Neuromodulator Neurons Noise Output Pattern Perception Play Population Dynamics Positioning Attribute Preparation Primates Probability Property Pupil Reaction Time Research Project Grants Resolution Rodent Role Satiation Sensory Signal Transduction Source Specific qualifier value Speed Stimulus Structure Technology Testing Text Thirst Time Training Typology Visual Perception Work Writing alertness area striata cell type data modeling design experimental study interest laboratory experiment molecular phenotype network models neural new technology nonhuman primate optogenetics predictive modeling sensory cortex sensory input theories tool vigilance visual stimulus

项目摘要

Research Project 1 - Internal state dynamics of perception and motivati0n Lead: Karl Deisseroth MD PhD Summary This project is focused on development and application of technology for probing the interaction of external sensory input with internal states of the mammalian brain. The unique leverage of our team is technology-driven experimental access to new regimes of speed, scope, and resolution in defining and tuning these internal states. First, we will quantify the population dynamics and statistical structure of time-varying spontaneous activity across the volume of primary visual cortex (with individual cells defined by position and wiring in 3D space, and by molecular identity) to test impact of spontaneous fluctuations on environmental stimulus-triggered neuronal activity and animal behavior. Next, we will quantify cortical activity during transitions among internal states (along with behaviors), impose precisely-defined ensemble activity to assess impact of state transitions on evoked population dynamics and behavior, and quantify endogenous activity influencing these states to assess natural mechanisms and effects. Finally, we will integrate our biological findings and new technological capabilities to directly and precisely impose more complex temporal dynamics of interest by controlling multiple single cells in multiple cortical regions over time. The same cells observed to be active and important in behavior will be made available for molecular phenotyping analysis, so that cell typology may be linked to the specific natural and causal roles of cells in this important domain of circuit computation and behavior.

研究项目 1 - 感知和动机的内部状态动态负责人：Karl Deisseroth 医学博士概括该项目的重点是探索外部相互作用的技术的开发和应用。哺乳动物大脑内部状态的感觉输入。我们团队的独特优势是技术驱动在定义和调整这些内部状态时，通过实验获得速度、范围和分辨率的新机制。首先，我们将量化随时间变化的自发活动的种群动态和统计结构跨越初级视觉皮层的体积（单个细胞由 3D 空间中的位置和接线定义，以及通过分子身份）来测试自发波动对环境刺激触发的神经元的影响活动和动物行为。接下来，我们将量化内部状态转换期间的皮质活动（以及行为），施加精确定义的集成活动来评估状态转换对诱发群体动态和行为，并量化影响这些状态的内源活动以评估自然机制和影响。最后，我们将整合我们的生物学发现和新技术通过控制多个来直接和精确地施加更复杂的感兴趣的时间动态的能力随着时间的推移，多个皮质区域的单细胞。观察到相同的细胞在行为中活跃且重要将可用于分子表型分析，以便细胞类型学可以与特定的细胞在电路计算和行为的这个重要领域中的自然和因果作用。