JAK-STAT Control of Zika Virus-Induced Fetal Injury

JAK-STAT 控制寨卡病毒引起的胎儿损伤

• 批准号: 10321508
• 负责人: KRISTINA M. ADAMS WALDORF
• 金额: $ 32.84万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-10 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10321508
• 关键词: 10 year old; Age; Biological Assay; Cells; Computer software; Contract Services; Dangerousness; Funding; Future; Goals; High-Throughput RNA Sequencing; Human; Hybrids; Injury; Libraries; Modeling; Pregnancy; Protocols documentation; Quality Control; RNA; Sampling; Viral; Virus; Work; Workload; ZIKV infection; Zika Virus; fetal; human tissue; immunoregulation; instrument; nonhuman primate; therapeutic development; viral transmission

Project Summary The current project, "Jak STAT control of Zika virus-induced fetal injury" (R01 AI143265), includes the processing of many nonhuman primate (NHP) and human tissue and cell samples for high throughput RNA sequencing (RNAseq) analysis. Our sample-processing pipeline for these assays includes RNA isolation and quality control (QC), and library construction and library QC. We currently utilize a Biomek FX automated workstation to perform the majority of these work steps in handling high volume samples in our high throughput workload for these analyses. The Biomek FX is 10 years old. Due to the age, it is not reliable, the ability to obtain a future service contract is not guaranteed, and the current configuration and software are not compatible with off- the-shelf protocols. Thus, we are in need of funds to replace this instrument. The purchase of a Biomek i7 Hybrid automated workstation to replace our Biomek FX workstation, allows us to achieve our stated project goals. 0

项目摘要 当前的项目“ Zika病毒引起的胎儿损伤的JAK Stat Control”（R01 AI143265）包括 许多非人类灵长类动物（NHP）以及人体组织和细胞样品的加工 吞吐量RNA测序（RNASEQ）分析。我们为这些的样品处理管道 测定包括RNA隔离和质量控制（QC），以及图书馆的构建和图书馆QC。 我们目前使用Biomek FX自动化工作站来执行这些工作的大多数 处理这些分析的高吞吐量工作负载中的大量样品的步骤。这 Biomek FX已有10年的历史。由于年龄，这是不可靠的，可以获得未来服务的能力 不能保证合同，当前的配置和软件与非兼容 搁置协议。因此，我们需要资金来替换该工具。购买 Biomek i7混合自动化工作站以替换我们的Biomek FX工作站，使我们能够 实现我们既定的项目目标。 0