WNPRC STEM CELL RESOURCE

WNPRC干细胞资源

• 批准号: 7958782
• 负责人: James Alexander Thomson
• 金额: $ 9.38万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958782
• 关键词: Callithrix; Cell Culture Techniques; Cell Line; Cells; Communication; Communities; Computer Retrieval of Information on Scientific Projects Database; Culture Techniques; Derivation procedure; Disease; ES Cell Line; Embryo; Fibroblast Growth Factor 2; Freezing; Funding; Future; Generations; Grant; Institution; Journals; Knowledge; Macaca mulatta; Nature; Paper; Patients; Peer Review; Plasmids; Primates; Proteins; Publications; Publishing; Recombinant Proteins; Reporting; Research; Research Institute; Research Personnel; Resource Allocation; Resources; Rosa; Services; Source; Spinal Muscular Atrophy; Stem Cell Research; Stem cells; Time; Training; United States National Institutes of Health; Virginia; Wisconsin; Work; Zebrafish; base; embryonic stem cell; induced pluripotent stem cell; interest; investigator training; journal article; meetings; member; plasmid DNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To maintain and further develop a specialized resource for studies relating to embryonic stem cell research. Allocation of Resource Access To date, the stem cell resource unit at the Wisconsin National Primate Research Center provides frozen rhesus and marmoset ES cells to interested investigators. No request has been denied. Additionally, the stem cell resource unit provides zebrafish bFGF for culturing primate ES cells. Since last submission 4 new investigators have received the recombinant protein bringing the total number of investigators receiving zbFGF on a regular basis to 18. Over 250mg of zbFGF has been distributed to date. This has saved investigators over $700,000. The DNA plasmid used to purify the protein itself is now available through Addgene (www.addgene.com) and was sent to 18 new investigators in 2008 bringing the total number of investigators to receive this plasmid to 26. Lastly, 4 investigators requested and received rhesus ES cell provided by the stem cell resources unit and distributed by the WiCell Research Institute. Dissemination Knowledge is disseminated to the scientific community via publications in peer reviewed journals and scientific meeting attendance. The Wisconsin National Primate Research Center also holds quarterly research retreats to create increased communication between the various service and resource units. Training Training in culture techniques of primate embryonic stem cells is available. Many new investigators have taken advantage of this resource in previous reporting periods however there have been no new investigators trained this year. Progress We have provided ips cell derivation for disease specific cell lines for UW investigators. To date, 3 disease cell lines have been used for ips cell generation and a total of 29 clones have been cultured and frozen for future use. Highlights: Members of stem cell resources Yu and Thomson were authors on a paper detailing reprogramming in cell cultured from a donor afflicted with a specific disease: Allison D. Ebert, Junying Yu, Ferrill F. Rose, Jr, Virginia B. Mattis, Christian L. Lorson, James A. Thomson, & Clive N. Svendsen. Induced pluripotent stem cells from a spinal muscular atrophy patient. Nature 457, 277-280 (15 January 2009) Published online 21 December 2008. Challenges: Due to funding shortages we were unable to receive cynomologous embryos and the project came to a halt. We look forward to working with CPI to receive Mauritian cynomolougous embryos to create new cynomologous ES cell lines as described in our P51 proposal. Concerns: No concerns at this time. Publications note: Stem Cell Resource support is involved in numerous journal articles that depend in part or in full on WNPRC resources.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 目的：维护并进一步开发胚胎干细胞研究相关的专门资源。 资源访问的分配 迄今为止，威斯康星国家灵长类动物研究中心的干细胞资源部门向感兴趣的研究人员提供冷冻恒河猴和狨猴 ES 细胞。没有任何请求被拒绝。此外，干细胞资源单元还提供斑马鱼 bFGF 用于培养灵长类 ES 细胞。自上次提交以来，已有 4 名新研究人员接受了重组蛋白，使定期接受 zbFGF 的研究人员总数达到 18 人。迄今为止，已分发了超过 250 毫克的 zbFGF。这为调查人员节省了超过 700,000 美元。用于纯化蛋白质本身的 DNA 质粒现在可通过 Addgene (www.addgene.com) 获得，并于 2008 年发送给 18 名新研究人员，使接收该质粒的研究人员总数达到 26 人。最后，4 名研究人员请求并收到恒河猴ES细胞由干细胞资源单位提供并由WiCell研究所分配。 传播 知识通过同行评审期刊上的出版物和出席科学会议向科学界传播。威斯康星国家灵长类动物研究中心还每季度举办一次研究静修活动，以加强各个服务和资源单位之间的沟通。 训练 提供灵长类胚胎干细胞培养技术培训。许多新调查员在之前的报告期间都利用了这一资源，但今年没有培训新调查员。 进步 我们为华盛顿大学研究人员提供了疾病特异性细胞系的 ips 细胞衍生。迄今为止，已使用 3 个疾病细胞系进行 ips 细胞生成，并培养和冷冻了总共 29 个克隆以供将来使用。 亮点： 干细胞资源组织的成员 Yu 和 Thomson 撰写了一篇论文，详细介绍了从患有特定疾病的捐赠者身上培养的细胞的重编程：Allison D. Ebert、Junying Yu、Ferrill F. Rose, Jr、Virginia B. Mattis、Christian L.洛森、詹姆斯·A·汤姆森和克莱夫·N·斯文森。来自脊髓性肌萎缩症患者的诱导多能干细胞。 Nature 457, 277-280（2009 年 1 月 15 日）2008 年 12 月 21 日在线发布。 挑战： 由于资金短缺，我们无法接收食蟹猴胚胎，该项目被迫停止。我们期待与 CPI 合作，接收毛里求斯食蟹猴胚胎，以创建新的食蟹猴 ES 细胞系，如我们 P51 提案中所述。 担忧： 目前无需担心。 出版物说明：许多期刊文章都涉及干细胞资源支持，这些文章部分或全部依赖于 WNPRC 资源。