THE PRIMATE EMBRYO GENE EXPRESSION RESOURCE

灵长类胚胎基因表达资源

• 批准号: 7959035
• 负责人: Keith E Latham
• 金额: $ 7.12万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959035
• 关键词: Animal Model; Basic Science; California; Cells; Clinical; Computer Retrieval of Information on Scientific Projects Database; Databases; Dot Immunoblotting; Embryo; Embryology; Evaluation; Fertilization; Funding; Gene Expression; Grant; In Vitro; Institution; Libraries; Macaca mulatta; Modeling; Molecular; Oocytes; Ovarian; Primates; Procedures; Research; Research Personnel; Resources; Sampling; Scientist; Source; Staging; Stem cells; Training; United States National Institutes of Health; blastocyst; cDNA Library; clinical effect; clinical practice; cost; hatching; in vivo; novel; tool; web site

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Primate Embryo Gene Expression Resource permits the rapid and cost-effective study of gene expression in primate oocytes and preimplantation embryos using the rhesus monkey as the primary model organism. PREGER contains over 200 informative samples representing GV-stage oocytes, MIIstage oocytes, embryos at the 1-cell through hatched blastocyst stages, obtained using different combinations of in vitro or in vivo maturation, fertilization, and culture. The resource also contains stem cells and ovarian cells. This extensive and valuable sample set permits analyses of gene expression related to understanding basic primate embryology, as well as potential effects of clinical procedures on embryo quality using a primate model. Dot blots for expression analysis, cDNA libraries, probes, and other tools in PREGER can be distributed to other scientists, providing unprecedented opportunities for molecular studies in primate embryos. The libraries can be employed for a myriad of other purposes. The resource also includes a web site and online database. Most recently, the resource has incorporated an annual molecular embryology training course. The resource will facilitate basic research, and may support novel clinical approaches, e.g., in the evaluation of embryo quality. The proposed activities will at once maintain and expand the resource, produce new and fundamental basic information about primate embryos, and produce novel molecular approaches that may be useful in clinical practice.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 灵长类动物的胚胎基因表达资源允许使用恒河猴作为主要模型有机体对灵长类动物卵母细胞和植入前胚胎的基因表达进行快速和成本效益的研究。 Preger包含200多个参考的样品，这些样本通过孵化的胚泡阶段，在1细胞的1细胞处，在1细胞的胚胎中，使用不同的体外或体内成熟，施肥和培养物获得。 该资源还包含干细胞和卵巢细胞。 这种广泛而有价值的样本集可以分析与理解基本灵长类动物胚胎学以及使用灵长类动物模型对胚胎质量的潜在影响有关的基因表达。用于表达分析的DOT印迹，cDNA文库，探针和其他工具可以分配给其他科学家，从而为灵长类动物胚胎的分子研究提供了前所未有的机会。图书馆可以用于无数其他目的。资源还包括一个网站和在线数据库。 最近，该资源纳入了年度分子胚胎学培训课程。 该资源将促进基础研究，并可能支持新颖的临床方法，例如评估胚胎质量。拟议的活动将立即维持和扩展资源，生成有关灵长类动物胚胎的新的基本基本信息，并产生可能在临床实践中有用的新型分子方法。