Role of the central nucleus of the amygdala during ethanol-rewarded instrumental tasks

杏仁核中央核在乙醇奖励的仪器任务中的作用

• 批准号: 10679383
• 负责人: Matilde Castro
• 金额: $ 4.77万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10679383
• 关键词: Affect; Alcohol consumption; Alcohols; Amygdaloid structure; Anatomy; Animal Behavior; Animals; Attention; Automobile Driving; Behavior; Behavioral; Cell Nucleus; Chronic; Communication; Consumption; Cues; Data; Dependence; Electrodes; Electrophysiology (science); Ethanol; Event; Food; Glass; Implanted Electrodes; Individual; Intake; Intervention; Intoxication; Learning; Mediating; Medical; Monitor; Motivation; Neurobiology; Neurons; Nutrient; Oral; Organism; Output; Pattern; Procedures; Process; Ramp; Rattus; Research; Research Personnel; Rewards; Role; Satiation; Science; Self Administration; Sodium; Substance Use Disorder; Techniques; Testing; Thirst; Time; Training; Translating; Volatilization; Water; Work; Writing; alcohol research; alcohol reward; alcohol seeking behavior; career; drinking; experience; experimental study; improved; in vivo; motivated behavior; motor behavior; neural; neural correlate; neurophysiology; novel therapeutics; optogenetics; pharmacologic; preference; programs; recruit; reinforcer; response; reward processing; skills

PROJECT SUMMARY The motivation to consume ethanol is a fickle thing. It differs wildly between individuals, but even in one individual, this motivation is not always constant. However, the exact dynamics that translate motivation to consume to the literal act of consumption remain poorly understood. The central nucleus of the amygdala may be an essential piece in this process. Its anatomical connections hint at its potential role in ethanol and food reward consumption, given that it is densely innervated by canonical reward processing centers and itself innervates regions directing motor behaviors and attention. However, the exact mechanisms and firing patterns that drive the central nucleus of the amygdala’s involvement in driving reward consumption remain poorly understood. This proposal makes use of a foundational technique, in vivo electrophysiology, to uncover how the central nucleus of the amygdala electrical activity relates to alcohol seeking and taking. In looking at the activity of this region over the course of a whole instrumental task session, a clearer conclusion about the central nucleus of the amygdala’s role in synthesizing motivation to drive motor behaviors can be obtained. In aim 1, the neural activity pattern in the central nucleus of the amygdala will be monitored over the course of an ethanol self-administration task to understand how activity in this region changes across trials as an animal’s motivation to consume ethanol wanes. For aim 2, the proposed electrophysiology recordings will occur when water-deprived rats perform a choice task that promotes a switch in reward preference from water to ethanol. These planned recordings will elucidate central nucleus of the amygdala activity when there are competing motivations. In aim 3, the effect of dependence on both instrumental tasks will be assessed to understand how the firing in this region following dependence-inducing high concentrations of ethanol. In completing these aims and disseminating the data I find, I will also further my independence and confidence as an independent researcher while also improving my theoretical, technical, and professional skills. Overall, this project aims to understand the central nucleus of the amygdala’s role as a nexus point between reward seeking and reward consumption. This would not only deepen our understanding of this nucleus’s role as a whole, but could potentially contribute to novel therapeutic behavioral and medical interventions.

项目概要 消费乙醇的动机是一件变化无常的事情，个体之间差异很大，但即使在一个人身上也是如此。 对于个人来说，这种动机并不总是恒定的，但是，将动机转化为具体的动力。 杏仁核的中央核可能对字面上的消费行为了解甚少。 它的解剖学联系暗示了它在乙醇和食品中的潜在作用。 奖励消费，因为它受到规范奖励处理中心及其本身的密集支配 神经支配指导运动行为和注意力的区域然而，确切的机制和放电模式。 驱动杏仁核参与奖励驱动消费的中央核仍然很差 该提案利用体内电生理学这一基础技术来揭示其中的原理。 杏仁核的中央核电活动与寻找和摄取酒精有关。 该区域在整个工具性任务会议过程中的活动，关于 可以获得杏仁核在合成动机以驱动运动行为中的作用。 目标 1，将在一个过程中监测杏仁核中央核的神经活动模式 乙醇自我管理任务，以了解该区域的活动在动物实验中如何变化 对于目标 2，建议的电生理学记录将在以下情况发生。 缺水的老鼠执行一项选择任务，促进奖励偏好从水转向乙醇。 这些计划的记录将阐明当存在竞争时杏仁核活动的中央核 在目标 3 中，将评估对两项工具性任务的依赖的影响，以了解其影响。 为了完成这些目标，在导致依赖的高浓度乙醇之后在该地区进行了燃烧。 并传播我发现的数据，我也将进一步增强我作为独立人士的独立性和信心 总体而言，该项目旨在提高我的理论、技术和专业技能。 了解杏仁核的中央核作为奖励寻求和奖励之间的连接点的作用 这不仅会加深我们对这个核心的整体作用的理解，而且可以。 可能有助于新的治疗行为和医疗干预措施。