Single-Cell Biology Shared Resource

单细胞生物学共享资源

基本信息

批准号：
10675645
负责人：
Jesse Gillis
金额：
$ 20.96万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10675645
关键词：
ATAC-seq Bioinformatics Cancer Center Cancer Center Support Grant Cancer Center at Cold Spring Harbor Laboratory Capital Cell Culture Techniques Cell Nucleus Cell Separation Cells Cellular biology Communities Complex Copy Number Polymorphism Custom Data Data Analyses Data Set Dissociation Doctor of Philosophy Educational workshop Equipment Evolution Experimental Designs Faculty Gene Expression Gene Expression Profiling Generations Genomics Genotype Head Heterogeneity Hour Human Resources Immune response Immunologic Receptors Informatics Investigation Laboratories Learning Libraries Lipids Logistics Malignant Neoplasms Manuscripts Mission Mutation Population Preparation Process Proteins Protocols documentation Publications Quality Control Reagent Research Research Personnel Resolution Resource Sharing Resources Sampling Services Side Techniques Technology Tissues Training and Education Work cell type community center computational pipelines computerized data processing design digital genomic data member novel programs proteogenomics protocol development single cell analysis single cell sequencing tool transcriptome transcriptome sequencing transcriptomic profiling transcriptomics tumor heterogeneity tumor microenvironment tumor progression

项目摘要

SINGLE-CELL BIOLOGY SHARED RESOURCE – PROJECT SUMMARY/ABSTRACT The Single-Cell Biology Shared Resource was founded in 2017 with the mission of pioneering and deploying cutting-edge single-cell genomics technologies to members of the CSHL Cancer Center and surrounding research community. The Shared Resource is led by Faculty Head Jesse Gilis, Ph.D., and Director Jonathan Preall, Ph.D. The Single-Cell Biology Shared Resource is designed to be a comprehensive, collaborative Shared Resource facility that supports every stage of a single-cell genomics project, including experimental design, protocol development, sample preparation, library construction, sequencing logistics, primary data processing, and in-depth embedded analysis. Shared Resource personnel frequently work side-by-side with researchers, customizing the workflow to meet the unique needs of the project. Examples of the most common single-cell workflows supported by the Shared Resource include: 3’-digital transcriptome profiling, immune receptor profiling, ATAC-sequencing, copy-number variation, proteogenomics, and single-nucleus RNAseq. In addition to standard, established protocols, the Shared Resource assists users in designing and executing custom single- cell workflows with downstream tailored data analysis. The Single-Cell Biology Shared Resource houses and operates capital equipment for handling most stages of common single-cell workflows. A critical component of this Shared Resource’s mission is to provide education and training on the analysis of single-cell genomics data. Semi-annual workshops and regular computational office hours give researchers the opportunity to learn common computational pipelines and become familiar with emerging techniques in data analysis. Since its establishment, the Shared Resource has been used by 12 CSHL Cancer Center laboratories (32% of members), resulting in 5 co-authored publications. In summary, the Single-Cell Biology Shared Resource provides comprehensive bench-to-publication support of both off-the-shelf and customized genomics workflows, dramatically streamlining the process of collecting these challenging and high-impact datasets.

单细胞生物学共享资源 - 项目摘要/摘要单细胞生物学共享资源成立于2017年，其使命是开拓和部署向 CSHL 癌症中心及周边地区的成员提供尖端的单细胞基因组学技术共享资源由教务主任 Jesse Gilis 博士和主任 Jonathan 领导。 Preall 博士单细胞生物学共享资源旨在成为一个全面的协作共享资源支持单细胞基因组学项目各个阶段的资源设施，包括实验设计、方案开发、样品制备、文库构建、测序物流、初级数据处理、以及深入的嵌入式分析，共享资源人员经常与研究人员并肩工作，定制工作流程以满足项目的独特需求最常见的单细胞示例。共享资源支持的工作流程包括：3'-数字转录组分析、免疫受体分析、ATAC 测序、拷贝数变异、蛋白质组学和单核 RNAseq。标准的、既定的协议，共享资源可帮助用户设计和执行定制的单具有下游定制数据分析的细胞工作流程。操作用于处理常见单细胞工作流程的大多数阶段的资本设备。该共享资源的使命是提供有关单细胞基因组学数据分析的教育和培训。半年一次的研讨会和定期计算办公时间为研究人员提供了学习的机会通用计算管道并熟悉数据分析中的新兴技术。建立后，共享资源已被 12 个 CSHL 癌症中心实验室（占成员的 32%）使用，产生了 5 份共同撰写的出版物总之，单细胞生物学共享资源提供了。对现成的和定制的基因组学工作流程提供全面的从实验到发表的支持，极大地简化了收集这些具有挑战性和高影响力的数据集的过程。