Single Cell Characterization and Procurement Core

单电池表征和采购核心

基本信息

批准号：
10673652
负责人：
Marie-Dominique Filippi
金额：
$ 24.02万
依托单位：
CINCINNATI CHILDRENS HOSP MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-01 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10673652
关键词：
ATAC-seq Area Awareness Bioinformatics Biological Biological Assay Biological Process Biology Bone Marrow Cell Count Cell Differentiation process Cell Separation Cell physiology Cells Cellular biology Cities Classification Client Complex Consultations Core Facility Data Analyses Data Analytics Data Set Dedications Development Dimensions Disease Dissection Ensure Epigenetic Process Equipment Erythroid Cells Experimental Designs Flow Cytometry Fostering Genomics Goals Health Services Accessibility Hematology Hematopoiesis Hematopoietic Hematopoietic System Hematopoietic stem cells Heterogeneity Image Imaging Techniques Immune Immunofluorescence Immunologic Immunology Immunophenotyping In Situ Individual Institution Joints Lysosomes Measures Mitochondria Molecular Myelogenous National Institute of Diabetes and Digestive and Kidney Diseases Non-Malignant Organelles Population Population Heterogeneity Procedures Process Proteome Protocols documentation Research Research Design Research Personnel Research Project Grants Resolution Services Signal Transduction Standardization Systems Biology Techniques Technology Tissues Training Training and Education Translations Universities Wisconsin Work analysis pipeline biological systems cell preparation cellular imaging cost data analysis pipeline data integration design dimensional analysis epigenome experience imaging facilities implementation barriers innovation instrument intercellular communication large datasets microscopic imaging novel novel strategies progenitor programs single cell analysis single-cell RNA sequencing stem stem cell biology stem cells superresolution microscopy tool transcription factor transcriptome transcriptomics ultra high resolution

项目摘要

SCCPC Abstract As a direct result of our ability to analyze hematopoietic cells at the single cell level, our understanding of the complexity and heterogeneity of the hematopoietic system has dramatically evolved in the past 10 years. The use of high-end flow cytometry and transcriptomics allowed investigators to measure cell-to- cell differences and correlate genomic information with single-cell function. Our understanding of non- malignant hematology is rapidly evolving, and now requires integration of transcriptome, epigenome, proteome (and now “organellome”) both at the single-cell level and in bone marrow in situ. However, rigorous experimental dissection of hematopoietic stem and progenitors (HSCP) requires specialized expertise and fascile technical agility that serves as a barrier to many researchers in the field. To answer this critical need, the Single Cell Characterization and Procurement Core (SCCPC) is designed to provide both expertise on data analytics platforms as well as technical advise (and access to) specialized services to facilitate cutting-edge multi-dimensional single-cell analyses for functional and mechanistic understanding of non-malignant hematology. Although such technologies are widely available, access to these services at most institutions is not accompanied by requisite understanding of hematopoiesis and stem cell biology (and/or analysis pipelines). Thus, there is a need for easy access to training and expertise on using analytic platforms, and how to integrate datasets. The SCCPC provides unique services for investigating hematopoiesis at the single-cell level including: (1) standardized and centralized services in flow cytometry analysis and isolation to reduce costs for investigators; (2) specialized services in multispectral flow cytometry and multi-dimensional analyses; (3) specialized analysis pipelines for integrating orthogonal “omics” layers; (4) specialized services in high resolution confocal immunofluorescence imaging for fixed, live and in situ single cell imaging; (5) specialized services in study design, large dataset analyses and interpretation; and (6) development of new cutting-edge technologies for investigators to use in their research. The Central Goal of the SCCPC is to create a centralized center providing advise/expertise on data analyses and integration to facilitate a comprehensive cutting-edge multi-dimensional single-cell understanding – functionally and mechanistically – of non-malignant hematology. The SCCPC has dedicated professionally trained staff with great experience in scientific experimental design and client support. The aims of the core are to provide innovative, state-of-the-art facilities and sophisticated technical assistance for high quality analysis of single hematopoietic cells; to provide uniform quality of cell preparations; to reduce costs of individual investigators by using shared flow cytometry, genomics analyses and imaging facilities; and to train/educate in new concepts on cell analysis, isolation, bioinformatics analyses and imaging.

SCCPC摘要作为我们在单细胞水平上分析造血细胞能力的直接结果，我们的理解造血系统的复杂性和异质性在过去 10 年里发生了巨大的变化高端流式细胞术和转录组学的使用使研究人员能够测量细胞间的变化。细胞差异并将基因组信息与单细胞功能相关联。恶性血液学正在迅速发展，现在需要整合转录组、表观基因组、单细胞水平和骨髓原位的蛋白质组（现在的“细胞器组”）。造血干细胞和祖细胞 (HSCP) 的严格实验解剖需要专门的专业知识和复杂的技术敏捷性成为该领域许多研究人员回答的障碍。单细胞表征和采购核心 (SCCPC) 旨在满足这一迫切需求数据分析平台方面的专业知识以及技术建议（和获取）专业服务促进尖端的多维单细胞功能和机械分析尽管此类技术已广泛应用，但仍无法获得对非恶性血液学的了解。大多数机构的这些服务并不伴随着对造血作用和造血作用的必要了解。因此，需要轻松获得培训和分析。 SCCPC 提供了有关使用分析平台以及如何整合数据集的专业知识。单细胞水平的造血研究服务包括：（1）标准化和集中化（二）专业化服务多光谱流式细胞术和多维分析；（3）专门的分析管道整合正交“组学”层；(4) 高分辨率共焦专业服务；用于固定、活体和原位单细胞成像的免疫荧光成像 (5) 专业服务；研究设计、大型数据集分析和解释；(6) 开发新的前沿技术； SCCPC 的中心目标是创建一个供研究人员在研究中使用的技术。集中中心提供有关数据分析和集成的建议/专业知识，以促进全面的前沿多维单细胞理解——功能和从机制上来说，SCCPC 拥有经过专业培训的专门工作人员。在科学实验设计和客户支持方面拥有丰富的经验。提供创新、最先进的设施和先进的技术援助，以实现高质量分析单个造血细胞；提供统一质量的细胞制剂以降低成本；个别研究人员使用共享的流式细胞术、基因组学分析和成像设施；培训/教育细胞分析、分离、生物信息学分析和成像的新概念。