Kinesin in photoreceptors cells

感光细胞中的驱动蛋白

• 批准号: 7935221
• 负责人: DAVID S WILLIAMS
• 金额: $ 38.5万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2012-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7935221
• 关键词: Cell Culture Techniques; Cell Death; Cell physiology; Cells; Cellular biology; Characteristics; Cilia; Complex; Development; Distal; Genes; Genetic; Genetic Models; Goals; Growth; In Vitro; Kinesin; Kinetics; Knockout Mice; Lead; Life; Lighting; Link; Membrane Proteins; Microtubules; Modeling; Molecular; Motor; Movement; Mus; Mutant Strains Mice; Opsin; Photoreceptors; Physiological; Protein translocation; Proteins; RNA Interference; Retinal Diseases; Role; Suggestion; System; Testing; Transport Process; base; cellular imaging; experience; in vivo; inherited retinal degeneration; insight; kinetosome; protein complex; protein transport; research study; response; skills; tool

DESCRIPTION (provided by applicant): Kinesins are microtubule motor proteins that generate intracellular movement essential for many fundamental cellular processes. The long-term goal of this project is to understand the function of kinesin-2 in the photoreceptor cilium. The current application is based on three new developments: (1) A newly established model of opsin transport in cilia that can be studied by live-cell imaging; (2) Findings that link known retinal disease genes to kinesin-2 and ciliary transport; and (3) Refinement of the genetic model so that loss of kinesin-2 can be studied prior to photoreceptor cell death. We propose to capitalize upon these new findings and developments by: (1) Testing two competing hypotheses for how kinesin-2 generates opsin transport along the cilium, and testing the roles of different proteins in the transport of opsin along the photoreceptor cilium. (2) Determining the contribution of kinesin-2 in different (non-opsin) transport processes related to the photoreceptor cilium. The results of these studies will lead to a better understanding of critical cellular processes in the photoreceptor cilium, and thus provide important new insight into a major group of inherited retinal degenerations.

描述（由申请人提供）：动力素是微管运动蛋白，它们产生许多基本细胞过程必不可少的细胞内运动。该项目的长期目标是了解驱动蛋白2在感光细胞纤毛中的功能。当前的应用程序基于三个新的开发：（1）纤毛中新建立的Opsin Transport模型，可以通过活细胞成像研究； （2）将已知的视网膜疾病基因与驱动蛋白-2和睫状运输联系起来的发现； （3）遗传模型的细化，以便在感光细胞死亡之前可以研究驱动蛋白-2的丧失。我们建议通过以下方式利用这些新发现和发展，（1）测试两个相互竞争的假设，以实现驱动蛋白-2如何沿纤毛产生Opsin运输，并测试不同蛋白质在沿光感受器纤毛沿光蛋白运输中的作用。 （2）确定驱动蛋白-2在与感光纤毛相关的不同（非粘蛋白）传输过程中的贡献。这些研究的结果将导致对光感受器纤毛中关键细胞过程有更好的了解，从而为主要的遗传视网膜退化提供重要的新见解。