The Photoreceptor Cilium

光感受器纤毛

• 批准号: 9350346
• 负责人: DAVID S WILLIAMS
• 金额: $ 38.5万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9350346
• 关键词: Address; Animal Model; Biochemistry; Biological; Carrier Proteins; Cell Culture Techniques; Cell Line; Cell Survival; Cell membrane; Cell physiology; Cells; Cellular biology; Cilia; Diffusion; Disease; Elements; Endocytosis; Epithelial Cells; Event; Fluorescence Recovery After Photobleaching; Genes; Genetic Models; Goals; Golgi Apparatus; Guanosine Triphosphate Phosphohydrolases; Image; Impairment; Individual; Kinesin; Lateral; MYO7A gene; Measurement; Measures; Membrane; Membrane Proteins; Methods; Microscopy; Molecular; Movement; Mus; Mutant Strains Mice; Neck; Opsin; Pathogenesis; Phenotype; Photoreceptors; Process; Protein Sortings; Proteins; Research; Retinal Degeneration; Rhodopsin; Role; Route; Site; Study models; System; Testing; Time; Travel; Treatment Efficacy; Usher Proteins; Vesicle; Yeasts; base; ciliopathy; improved; innovation; insight; knock-down; live cell imaging; mutant; mutant mouse model; novel; preclinical study; prevent; public health relevance; retinal rods; trafficking

DESCRIPTION (provided by applicant): The photoreceptor outer segment is an elaborate primary cilium. The delivery of opsin into and along the cilium is critical for photoreceptor cell function and viability. The overall goal of this study is to understand the cellular mechanisms involved in these delivery processes. A combination of cell biological methods, including imaging of protein movements in the cilium of live mouse photoreceptor cells, will be used. Studies will address questions about opsin entry into and transport along the cilium, and the membrane diffusion barrier of the photoreceptor cilium. The proposed research incorporates technical innovation, including the measurements of real-time movements of opsin along the cilium, using a ciliated epithelial cell line and mouse rod photoreceptor cells. It will test novel concepts of opsin trafficking and the organization of the photoreceptor cilium.

描述（由申请人提供）：光感受器的外部段是精美的原发性纤毛。 OPSIN向纤毛的递送对于感光细胞功能和生存能力至关重要。这项研究的总体目标是了解这些输送过程中涉及的细胞机制。将使用细胞生物学方法的组合，包括使用活小鼠感光细胞纤毛中蛋白质运动的成像。研究将解决有关Opsin进入和运输纤毛的问题，以及光感受器纤毛的膜扩散屏障。拟议的研究结合了技术创新，包括使用纤毛上皮细胞系和小鼠杆光感受器细胞的Opsin沿Cilium的实时运动的测量。它将测试小说 OPSIN贩运的概念和感光细胞纤毛的组织。

项目成果

Ciliary transport of opsin.

视蛋白的纤毛运输。

• DOI: 10.1007/978-1-4419-1399-9_21
• 发表时间: 2010
• 期刊: Advances in experimental medicine and biology
• 作者: Trivedi,Deepti;Williams,DavidS
• 通讯作者: Williams,DavidS