Dissemination of MAPseq and BARseq for High-Throughput Brain Mapping

传播用于高通量脑图谱的 MAPseq 和 BARseq

基本信息

批准号：
10669012
负责人：
ANTHONY M ZADOR
金额：
$ 98.52万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2027-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10669012
关键词：
Acceleration Age Animal Model Animals Area Atlases Axon Bar Codes Biological Models Brain Brain Mapping Brain region Carrier Proteins Collaborations Communities Complex Computer software DNA sequencing Data Dedications Development Distant Engineering Foundations Gender Gene Expression Generations Genes Genetic Models Gilles de la Tourette syndrome Goals High-Throughput DNA Sequencing In Situ Infection Interneurons Label Laboratories Libraries Maps Mental Depression Methods Multiplexed Analysis of Projections by Sequencing Mus Neocortex Neuroanatomy Neurofibrillary Tangles Neurons Neuropil Neurosciences Neurosciences Research Population Presynaptic Terminals Probability Protocols documentation Publications Publishing RNA Reagent Resolution Reverse Transcription Role Schizophrenia Scientist Specificity Standardization Synapses Techniques Technology Time Training and Education Viral Virus Virus Replication Vision Work addiction anatomical tracing autism spectrum disorder bioinformatics pipeline brain tissue cost in situ sequencing insight interest neocortical neural neuronal circuitry neuropsychiatric disorder neurotransmission next generation next generation sequencing novel novel strategies skills tissue processing transcriptomics user-friendly

项目摘要

The goal of this project is to disseminate MAPseq and BARseq to the broader neuroscience community. These are novel methods developed in my laboratory based on high-throughput DNA sequencing for determining neuronal circuitry. Neurons transmit information to distant brain regions via long-range axonal projections. In some cases, functionally distinct populations of neurons are intermingled within a small region. Disruptions of connectivity may underlie many neuropsychiatric disorders including autism and schizophrenia. At present, neuroanatomical techniques—particularly those with single neuron resolution—are expensive and labor intensive. MAPseq and BARseq convert neuroanatomy into a problem of DNA sequencing, and thereby allow us to exploit the tremendous breakthroughs in next- generation sequencing throughput. The dissemination of these high-throughput methods for determining neuronal projections will have important implications for understanding normal neuronal circuitry, and how this circuitry is disrupted in animal models of neuropsychiatric disorders like autism and schizophrenia.

该项目的目标是将 MAPseq 和 BARseq 传播到更广泛的神经科学领域这些是我的实验室基于高通量 DNA 开发的新颖方法。确定神经元回路的测序。在某些情况下，通过长程轴突投影来区分功能不同的群体。神经元在一个小区域内混合在一起，连接中断可能是许多问题的根源。神经精神疾病，包括自闭症和精神分裂症。目前，神经解剖技术，特别是那些具有单神经元分辨率的技术， MAPseq 和 BARseq 将神经解剖学转化为一个昂贵且劳动密集型的问题。 DNA 测序，从而使我们能够利用下一步的巨大突破—— 这些高通量方法的传播。确定神经投射将对理解正常现象产生重要影响神经元回路，以及神经精神动物模型中该回路如何被破坏自闭症和精神分裂症等疾病。