Component: MHC Genetic Core

成分：MHC 遗传核心

基本信息

批准号：
10666558
负责人：
Amanda Vinson
金额：
$ 7.45万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10666558
关键词：
Acquired Immunodeficiency Syndrome Address Adult Age Algorithms Alleles Animal Husbandry Animal Model Animals Award Base Sequence Biological Assay Biomedical Research Breeding Caring Dedications Development Disease Disease Progression Ensure Female Gene Frequency Genetic Genetic Variation Genome Genomics Genotype Goals HIV Health Immigrant Individual Infant Informatics Knowledge Lead MHC Class I Genes Macaca Macaca mulatta Maintenance Morbidity - disease rate Practice Management Primates Protocols documentation Recommendation Reporting Research Research Support SNP genotyping Technology Time Uncertainty Work animal breeding animal colony cost experience feasibility testing genetic pedigree genetic testing genome-wide human disease improved male member mortality novel offspring preservation recruit simian human immunodeficiency virus success trait working group

项目摘要

PROJECT SUMMARY, Core C: MHC Genetic Typing Core Genetically well-characterized animal models are critical for the success of HIV/SHIV research, including characterization for MHC genotype, known ancestry, and pedigree relationships. In particular, informative and accurate MHC genotyping is essential, due to the complexity of the MHC class I loci in macaques, and the influence of allele type on disease progression. The need for genetically well-characterized animal models for research must also be balanced against the need to preserve genetic diversity in animal colonies dedicated to supporting biomedical research. This is a requirement not only to ensure long-term colony health, but to ensure the relevance of experimental findings to human disease. The ONPRC is a leader in the development in many of the current recommended approaches and technologies used in genetic typing and management of NHP colonies. The aims of the Genetics Core for this project are to 1) use state-of-the-art genetic testing to produce colony offspring of known parentage, ancestry, and MHC genotype and 2) to maintain the genetic diversity of this extended SPF colony while producing sufficient numbers of offspring to meet research needs. We will continue to use the Fluidigm 96-SNP parentage array to assign parentage. All new colony offspring will be typed for MHC Class I alleles using a sequence-based expressed allele assay. We will also conduct genetic management using new protocols developed to assign genetic value for each animal, and to propose breeding groups that will minimize the loss of genetic diversity in the colony. Lastly, we will develop new informatics protocols to support these efforts, including the consideration of MHC type in the maintenance of genetic diversity.

项目摘要，核心 C：MHC 基因分型核心遗传特征良好的动物模型对于 HIV/SHIV 研究的成功至关重要，包括 MHC 基因型、已知血统和谱系关系的表征。特别是信息丰富且由于猕猴中 MHC I 类基因座的复杂性，准确的 MHC 基因分型至关重要等位基因类型对疾病进展的影响。需要具有良好遗传特征的动物模型研究还必须与保护动物群体遗传多样性的需要相平衡支持生物医学研究。这不仅是为了确保蜂群的长期健康，也是为了确保实验结果与人类疾病的相关性。 ONPRC 是许多领域发展的领导者目前推荐用于 NHP 基因分型和管理的方法和技术殖民地。该项目遗传学核心的目标是 1) 使用最先进的基因测试来生产已知亲子关系、祖先和 MHC 基因型的群体后代，以及 2) 维持遗传多样性这个扩展的 SPF 群体同时产生足够数量的后代以满足研究需求。我们将继续使用 Fluidigm 96-SNP 亲子关系阵列来分配亲子关系。所有新的殖民地后代都将使用基于序列的表达等位基因测定对 MHC I 类等位基因进行分型。我们还将进行遗传使用新协议进行管理，为每只动物分配遗传价值，并提出育种建议群体，将最大限度地减少群体遗传多样性的损失。最后，我们将开发新的信息学支持这些努力的协议，包括在维持遗传性方面考虑 MHC 类型多样性。