FUNDAMENTAL CRYOBIOLOGY OF MACAQUE SPERMATOZOA

猕猴精子的基础低温生物学

• 批准号: 7715573
• 负责人: STUART A MEYERS
• 金额: $ 5.42万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715573
• 关键词: Alcohol dependence; Artificial Insemination; Biological Preservation; Communicable Diseases; Computer Retrieval of Information on Scientific Projects Database; Contraceptive methods; Cryopreservation; Development; Fertilization in Vitro; Funding; Genotype; Goals; Grant; Human; Individual; Infertility; Institution; Lead; Macaca; Macaca mulatta; Methods; Mission; Modeling; National Center for Research Resources; Pharmaceutical Preparations; Physiological; Population; Regenerative Medicine; Research; Research Personnel; Resources; Semen Donor; Seminal fluid; Source; Transgenic Organisms; United States National Institutes of Health; base; cryobiology; desire; embryonic stem cell; genetic resource; human disease; male; nervous system disorder; nonhuman primate; programs; response; somatic cell nuclear transfer; sperm cell; success; vaccine development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: Efficient preservation of genetic resources of the rhesus monkey (Macaca mullatta) is critical to the mission of the National Center for Research Resources. This application is aimed at the development of efficient macaque sperm cryopreservation and will aid in successful propagation of germplasm through artificial insemination and in vitro fertilization programs. Non-human primates (NHPs), especially genetically unique macaques, are important models of human disease with applications to infertility and contraception, infectious diseases and vaccine development, drug and alcohol addiction, neurological disorders, and regenerative medicine. Similar to human sperm, cryopreserved sperm from NHP's show variable cryoprotection success depending on the individual sperm donor. Semen donors are often selected based on successful cryopreservation results, however, many individual males, including rhesus males in research programs, have semen quality that fails to survive cryopreservation. This is likely to be particularly true concerning NHPs generated from embryonic stem cells, somatic cell nuclear transfer, and transgenics where propagation of a specific individual's genotype is desired. This grant analyzes some of the mechanisms that may cause poor cryoperformance and focuses on methods to uniformly cryopreserve these bimodal populations. Our central hypothesis is that an understanding of cryopreservation-associated physiological responses in sperm will lead to improvements in sperm cryosurvival. Our long-term goal is to develop an understanding of crucial mechanisms involved in cryopreservation of sperm in order to optimally preserve sperm from a variety of genotypically unique and valuable NHP males.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目的：有效地保存恒河猴（Macaca Mullatta）的遗传资源对于国家研究资源中心的任务至关重要。该应用旨在开发有效的猕猴精子冷冻保存，并有助于通过人工授精和体外受精计划成功地传播种质。非人类灵长类动物（NHP），尤其是遗传学独特的猕猴，是人类疾病的重要模型，在不育和避孕，传染病和疫苗发育，药物和酒精成瘾，神经系统疾病和再生医学中应用。与人类精子类似，NHP的冷冻保存精子显示出可变的冷冻保护成功，具体取决于单个精子供体。经常根据成功的冷冻保存结果选择精液供体，但是，许多男性，包括研究计划中的恒河猴，具有无法在冷冻保存中幸存下来的精液质量。这对于从胚胎干细胞，体细胞核转移以及需要特定个体基因型传播的转基因产生的NHP尤其如此。该赠款分析了一些可能导致冷冻性能不良的机制，并专注于统一冷冻ression这些双峰种群的方法。我们的核心假设是，对精子中与冷冻保存相关的生理反应的理解将导致精子冷冻生存的改善。我们的长期目标是了解对精子冷冻保存涉及的关键机制的理解，以便从各种基因型独特且有价值的NHP雄性中最好地保留精子。