Core 2

核心2

基本信息

批准号：
10631163
负责人：
Kavita Madhav Dhodapkar
金额：
$ 37.36万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-01 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10631163
关键词：
Anatomy Archives Bioinformatics Biological Biological Assay Biopsy Biostatistics Core Blood Cells Cellular Indexing of Transcriptomes and Epitopes by Sequencing Clinical Clinical Trials Communication Consent Correlative Study Coupled Culture Media Cytometry Data Database Management Systems Databases Diagnosis Ensure Ethics Evaluation Fluorescence Freezing Gene Expression Genomics Goals Histologic Histology Histopathology IACUC Image Immune Immune response Immune system Immunohistochemistry Immunologic Monitoring Immunologic Tests Immunologics Immunologist Immunotherapy Infrastructure Institutional Review Boards Investigation Liquid substance Lung Lung Adenocarcinoma Maintenance Malignant Neoplasms Malignant neoplasm of lung Molecular Immunology Monitor Mutation Nursing Research Pathologic Pathologist Pathology Patients Phenotype Procedures Process Proteomics Protocols documentation Quality Control Research Research Personnel Resistance Resource Sharing Resources STK11 gene Saliva Sampling Scientist Secure Services Slide Specific qualifier value Specimen Specimen Handling Stains Standardization Structure System Technical Expertise Tissue Banks Tissue Sample Tissues Translations Tumor Markers Tumor-infiltrating immune cells Update Work central database clinical practice clinical trial protocol data integration data sharing digital experience genomic data human tissue molecular pathology mouse model neoplastic preclinical study prospective repository single-cell RNA sequencing tool tumor

项目摘要

SUMMARY The goal of the Molecular Pathology and Immunology Core (Core 2) is to provide a centralized technical infrastructure, inclusive of materials and the technical expertise necessary to accomplish all tissue-based investigations including the collection of tissue samples, histopathological analysis, immune monitoring and maintenance of a centralized database. Core 2 will function as the main repository of patient specimens and will oversee specimen processing and histopathology. Collected tissue samples will be analyzed for P01 project specified tumor biomarkers and phenotyping of tumor immune infiltrates utilizing immunohistochemistry for tumor biomarkers and mass cytometry and single cell RNA sequencing for immune cell phenotyping. Two experienced lung pathologists and an expert immunologist will work closely with the Bioinformatics and Biostatistics Core 3 and the Administrative Core 1 to ensure coordinated procurement, archiving, and storage of human tissue samples (especially clinical trial specimens) and seamless integration of the data generated from tissue-based investigations, including from mouse models and clinical trials, into a secure, database for utilization and further analysis by Cores 1 and 3 and P01 projects. All tissue-based research will be performed with ethical research oversight from IRB and IACUC protocols. Continuous communication between the clinicians, scientists, research nurses, biostatisticians, bioinformaticians and pathologists together with established standardized operating procedures for all core activities will provide optimal tissue collection, processing, analysis, and data sharing for all projects.

概括分子病理学和免疫学核心（核心 2）的目标是提供集中的技术基础设施，包括完成所有基于组织的工作所需的材料和技术专业知识研究包括组织样本的收集、组织病理学分析、免疫监测和维护集中式数据库。核心 2 将作为患者标本的主要存储库将监督标本处理和组织病理学。将分析收集的组织样本中的 P01 利用免疫组织化学预测特定的肿瘤生物标志物和肿瘤免疫浸润的表型用于肿瘤生物标志物以及用于免疫细胞表型分析的质谱流式分析和单细胞 RNA 测序。二经验丰富的肺病理学家和免疫专家将与生物信息学和免疫学专家密切合作生物统计核心 3 和管理核心 1，确保协调采购、归档和存储人体组织样本（尤其是临床试验样本）的分析和生成数据的无缝集成从基于组织的研究（包括小鼠模型和临床试验）转化为安全的数据库核心 1 和 3 以及 P01 项目的利用和进一步分析。将进行所有基于组织的研究接受 IRB 和 IACUC 协议的伦理研究监督。之间的持续沟通临床医生、科学家、研究护士、生物统计学家、生物信息学家和病理学家以及为所有核心活动建立标准化操作程序将提供最佳的组织收集，所有项目的处理、分析和数据共享。