A Label-free Extracellular-vesicle Automated Purification System (LEAP System)

无标记细胞外囊泡自动纯化系统（LEAP系统）

基本信息

批准号：
10546042
负责人：
Yuchao Chen
金额：
$ 74.96万
依托单位：
WELLSIM BIOMEDICAL TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10546042
关键词：
Aliquot Automation Biological Markers Biological Sciences Biomedical Technology Blood Plasma Volume Cells Clinic Clinical Complex Computer software Consumption Culture Media Development Devices Diagnosis Disease Early Diagnosis Encapsulated Goals Intervention Investigation Label Laboratories Lipid Bilayers Lipids Manuals Membrane Methodology Methods Microfluidics Molecular Sieve Chromatography Nanoporous Nucleic Acids Outcome Performance Phase Plasma Population Process Proteins Recovery Research Research Personnel Running Saliva Sample Size Sampling Small Business Innovation Research Grant Source Speed System Techniques Technology Testing Time Translations Ultracentrifugation Urine base cell type cost cost effective design extracellular vesicles flexibility improved innovation interest nanoparticle new technology novel particle precision medicine pressure programs success tool

项目摘要

PROJECT SUMMARY / ABSTRACT Extracellular vesicles (EVs) are small lipid bilayer particles secreted by most cell types, which encapsulates biomolecules including lipids, proteins, and nucleic acids from cells of origin. Profiling of EV-derived biomarkers provides a promising approach for diagnosis of various diseases. However, one of the primary challenges for the research and application of EVs is a lack of a reliable method for efficient isolation of EVs and their subpopulations from complex biofluids. Researchers in this field suffer from tedious workflow, long processing time, high cost, and poor EV quality. To overcome the challenges, WellSIM proposes in this Phase II project, which built upon the promising results generated from our Phase I project, to complete development of a Label- free Extracellular-vesicle Automated Purification System (LEAP System) for isolation of total EVs or EV subpopulations from a variety of biofluids in a non-invasive and cost-effective manner. Once developed, the integrated LEAP System will offer excellent EV quality, throughput, and flexibility for EV isolation that can outperform ultracentrifugation and other isolation techniques.

项目概要/摘要细胞外囊泡 (EV) 是大多数细胞类型分泌的小型脂质双层颗粒，其封装生物分子，包括来自细胞来源的脂质、蛋白质和核酸。 EV 衍生生物标志物的分析为各种疾病的诊断提供了一种有前景的方法。然而，面临的主要挑战之一是电动汽车的研究和应用缺乏可靠的方法来有效隔离电动汽车及其来自复杂生物流体的亚群。该领域的研究人员面临工作流程繁琐、处理时间长的困扰时间长、成本高、电动汽车质量差。为了克服这些挑战，WellSIM 在第二阶段项目中提出，它建立在我们第一阶段项目产生的有希望的结果的基础上，完成标签的开发免费的细胞外囊泡自动纯化系统（LEAP 系统），用于分离总 EV 或 EV 以非侵入性且具有成本效益的方式从各种生物体液中提取亚群。一旦开发出来，集成的 LEAP 系统将为 EV 隔离提供卓越的 EV 质量、吞吐量和灵活性，优于超速离心和其他分离技术。