D1 AND D5 RECEPTOR SIGNALING IN MONKEY PFC

猴 PFC 中的 D1 和 D5 受体信号传导

• 批准号: 7715760
• 负责人: JILL RENEE' Glausier
• 金额: $ 2.85万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715760
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Dendrites; Dendritic Spines; Electrons; Elements; Family; Funding; Grant; Immunoprecipitation; Institution; Interneurons; Methods; Microscopic; Monkeys; Neuropil; Parvalbumins; Prefrontal Cortex; Presynaptic Terminals; Primates; Protein Isoforms; Protein phosphatase; Proteins; Receptor Signaling; Reporting; Research; Research Personnel; Resources; Signaling Protein; Source; Techniques; United States National Institutes of Health; calretinin; dopamine D5 receptor; inhibitor/antagonist; receptor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to use electron microscopic techniques to determine the localization of the D1 and D5 dopamine receptors and their signaling proteins within primate prefrontal cortex, and to identify any physical interactions between these receptors and their downstream effector proteins utilizing immunoprecipitation methods. The prefrontal cortex has a complicated circuitry, and understanding where these two receptors are located within specific elements of this circuitry will deepen our understanding of how D1 family receptor signaling effects prefrontal functioning. We have determined the localization of D1 and D5 in prefrontal cortex (PFC) neuropil of layers I, III and V; determined their co-localization within dendritic spines and axon terminals of layer III; and determined D1 and D5 localization to parvalbumin and calretinin interneuron dendrites and axon terminals. During the reporting period, we determined the neuropil localization of two signaling proteins, DARPP-32 and Inhibitor-1, in layer III of the primate PFC; and determined the localization of protein phosphatase-1 isoforms to parvalbumin interneurons. Moreover, we made significant progress in determining the localization of protein phosphatase-1 isoforms to calretinin interneurons.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目旨在使用电子显微镜技术来确定D1和D5多巴胺受体的定位及其信号传导蛋白在灵长类动物的前额叶皮层中，并确定这些受体及其下游效应蛋白之间利用免疫致敬方法的任何物理相互作用。 前额叶皮层具有复杂的电路，并且了解这两个受体位于该电路的特定元素中的位置将加深我们对D1家族受体信号如何效应前额叶功能的理解。 我们已经确定了D1和D5在层I，III和V层的前额叶皮层（PFC）中的定位。确定了它们在第三层的树突状刺和轴突末端中的共定位。并确定D1和D5定位于白蛋白细胞蛋白和钙蛋白素间神经元树突和轴突末端。 在报告期间，我们确定了在灵长类动物PFC的第三层中的两个信号蛋白Darpp-32和抑制剂1的神经定位。并确定蛋白质磷酸酶-1同工型在白蛋白中间神经元中的定位。 此外，我们在确定蛋白质磷酸酶-1同工型对钙蛋白素中间神经元的定位方面取得了重大进展。