TICK SALIVA INHIBITS INFLAMMATION IN MONOCYTES STIMULATED WITH B BURGDORFERI

蜱唾液抑制布氏 B 氏菌刺激的单核细胞炎症

• 批准号: 7716320
• 负责人: MARIO TOMAS PHILIPP
• 金额: $ 2.41万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-21 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716320
• 关键词: Antibodies; Black-legged Tick; Borrelia burgdorferi; Cells; Computer Retrieval of Information on Scientific Projects Database; Detection; Enzyme-Linked Immunosorbent Assay; Funding; Grant; Harvest; High Pressure Liquid Chromatography; Hour; Human; IL8 gene; Immune; In Vitro; Infection; Inflammation; Inflammatory; Inflammatory Response; Injury; Institution; Interleukin-6; Lead; Life; Mediator of activation protein; Methods; Pilocarpine; Process; Production; Research; Research Personnel; Resources; Saliva; Site; Source; Staining method; Stains; Ticks; Tissues; Trypan Blue; Tumor Necrosis Factor-alpha; United States National Institutes of Health; chemokine; concept; density; feeding; human TNF protein; monocyte; response; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Monocytes are important regulators of inflammation. They are able to produce pro-inflammatory mediators such as IL-8, a chemokine that controls the influx of mammalian inflammatory and immune cells to sites of injury and infection, and IL-6, a key B cell differentiation factor. The prolonged and tissue-damaging feeding process of ixodid ticks, in combination with bacterial transmission, should lead to a vigorous inflammatory response. However, it is believed that factors present in tick saliva may down-regulate such deleterious responses. We hypothesized that tick saliva would down-regulate pro-inflammatory mediators elicited in vitro from monocytes stimulated with B. burgdorferi. Methods: Cells of the human monocytic line THP-1 were seeded at an initial density of 5 x 105 /ml. Cells were stimulated with live B. burgdorferi strain B31-5A19 for 24 hours in the presence or absence of pilocarpine (PC)-induced I. scapularis saliva at 1:20 or 1:500 dilutions. Controls included PC-only treated cells. After stimulation, viability was assessed via Trypan Blue staining, and supernatants were harvested for IL-6, TNF-alpha, and IL-8 detection by antibody-sandwich ELISA. Results: Tick saliva (1:20) significantly inhibited production of IL-6, TNF-alpha, and IL-8 by THP-1 cells stimulated with B. burgdorferi when compared to B. burgdorferi-only treated cells. PC controls, at the final concentration present in tick saliva (0.7¿M), as determined by HPLC, had no effect. Conclusion: We have provided proof of concept that tick saliva inhibits monocyte dependent inflammatory responses to B. burgdorferi.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 单核细胞是炎症的重要调节剂。他们能够产生促炎性介质（例如IL-8），它是一种控制哺乳动物炎症和免疫细胞对损伤和感染部位的影响的趋化因子，以及关键B细胞分化因子IL-6。 ixodid壁虱的延长和组织损害的进食过程与细菌传播结合使用，应导致剧烈的炎症反应。但是，据信，唾液中存在的因素可能会下调这种有害的反应。我们假设tick唾液会下调从伯格多尔氏芽孢杆菌刺激的单核细胞中在体外引起的促炎性介质。方法：人单核细胞系THP-1的细胞以5 x 105 /ml的初始密度接种。在存在或不存在1:20或1：500稀释时，用活芽孢杆菌菌株B31-5A19刺激细胞24小时。对照包括纯PC处理的细胞。刺激后，通过锥形蓝染色评估生存力，并通过抗体 - 桑德威奇ELISA收集用于IL-6，TNF-Alpha和IL-8检测的上清液。结果：与纯b。仅一hurgdorferi治疗的细胞相比。由HPLC确定的tick唾液中的最终浓度（0.7€）的最终浓度无效。结论：我们提供了tick唾液抑制单核细胞对B. burgdorferi的炎症反应的概念证明。