Purchase of 900 MHz NMR Spectrometer

购买900 MHz核磁共振波谱仪

• 批准号: 7254697
• 负责人: DEBORAH S. WUTTKE
• 金额: $ 27.44万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-15 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7254697
• 关键词: Area; Arts; Basic Science; Biological; Biomedical Research; Censuses; Colorado; Communities; Complex; Country; DNA; Data; Development; Facility Construction Funding Category; Floor; Funding; Geographic state; Goals; Health Sciences; Home environment; Institution; International; Internet; Kansas; Location; Macromolecular Complexes; Maintenance; Medical center; Membrane Proteins; Methods; Micelles; Missouri; Modeling; NMR Spectroscopy; Nucleic Acids; Numbers; Online Systems; Personal Satisfaction; Proteins; RNA; Rain; Request for Proposals; Research; Research Personnel; Resolution; Review Committee; Roentgen Rays; Site; Solvents; Structural Biologist; Synchrotrons; Techniques; Texas; Time; United States; Universities; Utah; Viscosity; Writing; base; carbohydrate structure; design; experience; improved; instrument; instrumentation; macromolecule; medical schools; programs; research study; three dimensional structure

DESCRIPTION (provided by applicant): We propose to establish a Rocky Mountain Regional NMR Center with a 900 MHz NMR serving a large number of Universities in the Rocky Mountain and Central Regions. The NMR Center will be situated on the new Fitzsimons campus of the University of Colorado Health Sciences Center, and will serve a productive group of NMR structural biologists in the areas of protein, nucleic acid and carbohydrate structure and function. The user group consists of 19 researchers at 8 Institutions. Space in the new Basic Research Building at Fitzsimons has been specifically designed for the unique siting requirements of a 900 MHz NMR and construction of the building is well underway. Recent developments in TROSY-type experiments allow acquisition of extremely high resolution NMR spectra on much larger macromolecules than was previously possible. These TROSY experiments are optimized at ultra-high fields, and the requested state-of-the-art 900 MHz NMR spectrometer and cryoprobe will make it possible for the users to take advantage of these powerful new techniques. The primary goal of the Center will be to provide routine access to the unique capabilities of the 900 MHz NMR in order to accelerate their biological and biomedical research programs. In addition to acquiring their NMR data on-site, experienced users will be able to acquire 900 MHz NMR data over the Internet with the aid of facility staff. A second major goal of the NMR Center will be to establish and maintain a Web-based collaboratorium for sharing information and expertise in a set of six "focus areas." These focus areas are strengths of the user group and the collaboratorium will provide a valuable mechanism for experienced users to share their expertise with other users. The six initial focus areas for the NMR Center and Web collaboratorium are: Improved Resolution and Sensitivity for NMR Studies of Proteins; NMR Studies of Large Macromolecular Complexes; Studies of Protein and Nucleic Acid Dynamics; Improved Methods for NMR Studies of RNA and DNA; NMR Studies of Membrane Proteins; and Improved Spectral Resolution by Employing Reverse Micelles in Low Viscosity Solvents. A well designed organizational plan for administering the facility is presented which is modeled after plans at other established multi-user NMR and synchrotron facilities. Time on the 900 MHz NMR will be allocated based on written proposals which will be reviewed by a proposal review committee. NIH-funded research that requires the high field, high resolution, and unique capabilities of the 900 MHz NMR will be the highest priority for the center.

描述（由申请人提供）：我们建议建立一个落基山地区NMR中心，其900 MHz NMR在落基山和中部地区为大量大学提供服务。 NMR中心将位于科罗拉多大学健康科学中心的新Fitzsimons校园，并将在蛋白质，核酸和碳水化合物结构和功能的领域提供一组富有生产力的NMR结构生物学家。用户组由19个机构的19个研究人员组成。 Fitzsimons的新基础研究大楼中的空间专门为900 MHz NMR的独特选址要求而设计，建筑物的建设正在进行中。最新的Trosy型实验的发展允许在比以前更大的大分子上获取极高的分辨率NMR光谱。这些TROSY实验在超高场上进行了优化，并且要求的最先进的900 MHz NMR光谱仪和Cryrope将使用户有可能利用这些强大的新技术。该中心的主要目标是提供常规访问900 MHz NMR的独特功能，以加速其生物学和生物医学研究计划。除了在现场获取其NMR数据外，经验丰富的用户还可以在设施人员的帮助下通过Internet获取900 MHz NMR数据。 NMR中心的第二个主要目标是建立和维护基于Web的协作，以在六个“焦点领域”中共享信息和专业知识。这些焦点领域是用户组的优势，协作室将为有经验的用户提供与其他用户分享其专业知识的宝贵机制。 NMR中心和Web协作的六个初始焦点领域是：提高蛋白质NMR研究的分辨率和敏感性；大型大分子复合物的NMR研究；蛋白质和核酸动力学的研究；改进了RNA和DNA NMR研究的方法；膜蛋白的NMR研究；并通过在低粘度溶剂中使用反向胶束来改善光谱分辨率。 介绍了一个精心设计的组织计划，用于管理该设施，该计划是根据其他已建立的多用户NMR和同步器设施的计划进行建模的。将根据书面提案分配900 MHz NMR的时间，该提案将由提案审查委员会审查。 NIH资助的研究需要900 MHz NMR的高场，高分辨率和独特功能，这将是该中心的最高优先级。