Biochemistry Core

生物化学核心

• 批准号: 7652407
• 负责人: Quing Jiang
• 金额: $ 12.52万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7652407
• 关键词: 5-chlorouracil; Aging; Animals; Antioxidants; Arts; Biochemistry; Biological; Biological Assay; Biological Markers; Bromouracil; Comet Assay; Complement; Cultured Cells; DNA; DNA Damage; Detection; Dietary Intervention; Disease; Eicosanoids; Guanine; Health Services Research; High Pressure Liquid Chromatography; Human; Inflammation; Inflammatory; Injury; Laboratories; Lipid Peroxidation; Lipids; Liquid substance; Malnutrition; Measures; Metabolic; Methods; Nitric Oxide; Nutrient; Oxidants; Peroxidase; Plasma; Population; Process; Proteins; Publications; Research; Research Design; Role; Sampling; Site; Techniques; Technology; Tocopherols; Work; adduct; airway inflammation; assay development; base; cost; crosslink; design; eosinophil peroxidase; experience; gamma-Tocopherol; granulocyte; human disease; macromolecule; member; micronucleus; oxidation; tool

Dr. Ames will be directing the biochemistry core at CHORI which will carry out various assays in serum, plasma and airway fluid samples collected in the conduct of all three projects. Dr. Ames laboratory has extensive experience in bioassays and have developed numerous assays for biomarkers for oxidative damage and antioxidants. These assays include HPLC and GC-MS based detection of antioxidants and oxidized products in biological samples. The assays include detection of tocopherol, 5-Nitro-gamma-tocopherol (a stable adduct formed by oxidation of gamma-tocopherol), beta-CEHC (2,7,8-trimethyl-2-(beta-carboxyethyl)-6-hydroxychroman, a metabolite. They have also developed assays to assess levels of 5-Chlorouracil (5-CIU) and 5-Bromouracil (5-BrU), markers for the action of myeloperoxidase (MPO) and eosinophil peroxidase (EPO) from granulocytes involved in airway inflammation. The assays, mostly developed in the Ames lab, for detection of oxidative damage include lipid peroxidation (MDA assay, a GC-MS method), and DNA damage markers: AP sites, and comet assays, 8-oxo-Guanine, DNA-protein cross links, and double-strand breaks (micronuclei).

艾姆斯博士将指示Chori的生物化学核心，该核心将进行各种 在所有三个项目的进行中收集的血清，血浆和气道流体样品的测定。 AMES实验室博士在生物测定方面拥有丰富的经验，并为氧化损伤和抗氧化剂的生物标志物开发了许多测定法。这些测定包括基于HPLC和GC-MS在生物样品中对抗氧化剂和氧化产物的检测。该测定包括检测毒素，5-硝基γ-生育酚（由γ-生皮酚的氧化形成的稳定加合物），β-CEHC（2,7,8-三甲基-2-（2,7-三甲基-2-（β-羧乙基乙基）-6-羟基甲基）-CHORMAN-CREACER（他们还评估了5-- cror）。 5-溴化物（5-bru），骨髓过氧化物酶（MPO）和嗜酸性粒细胞过氧化物酶（EPO）的作用，涉及气道炎症的颗粒细胞，主要在AMES LAB中开发，用于检测氧化损伤的方法。和彗星测定法，8-氧气瓜氨酸，DNA-蛋白交叉链路和双链断裂（微核）。