CORE--Biomarker

核心--生物标志物

• 批准号: 7621044
• 负责人: John Francis Keaney
• 金额: $ 9.67万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7621044
• 关键词: Address; Animal Model; Animals; Antibodies; Biological Assay; Biological Markers; Clinical; Cultured Cells; Data; Disease; Enzymes; Goals; Housing; Human; Individual; Link; Measurement; Measures; Methods; Monitor; Patients; Plasma; Radioimmunoassay; Reporter; Resources; Sampling; Specimen; Translational Research; Urine; base; human disease; insight; programs; response; tissue culture

One of the most difficult components of clinical and translational research is the task of monitoring disease activity and developing insights into the mechanisms of disease. One well established method of addressing these issues is the measurement of biomarkers from human and animal specimens. The goal of this Core is to provide for the measurement of biomarkers in samples derived from the individual projects of this SCCOR program. In order to accomplish this goal, we will offer these individual projects a uniform resource for the large-scale measurement of biomarkers in plasma, serum, and urine. We will also use tissue culture methods to develop reporter assays that will employ the exposure of patient samples to cultured cells and measuring the evoked response. We will also centralize and organize the storage of samples from the individual projects so that samples are readily available for assay with any new or emerging biomarkers that become available during the course of this project. The principal means of measuring biomarkers in this core unit will be commercially available enzyme-linked immunoabsorbant assays (ELISAs) or radioimmunoassays (RIAs). Because some assays may not be commercially available, this core will also generate in house ELISA and RIA methods using commercially-available antibodies. This core unit will generate data that spans all projects of the SCCOR program and thus, should provide a common basis for comparison between animal models and human disease.

临床和转化研究最困难的组成部分之一是监测疾病的任务 活动并发展对疾病机制的见解。一种良好的解决方法 这些问题是对人类和动物标本的生物标志物的测量。这个核心的目标是 在此SCCOR的各个项目中提供的样品中的生物标志物测量 程序。为了实现这一目标，我们将为这些个人项目提供统一的资源 大规模测量血浆，血清和尿液中的生物标志物。我们还将使用组织培养 开发记者测定的方法，该测定法将采用患者样品暴露于培养的细胞和 测量诱发的响应。我们还将集中并组织样品的存储 单个项目，以便可以与任何新的或新兴的生物标志物一起进行测定 在此项目过程中可用。测量生物标志物的主要手段 核心单元将是商业上可用的酶连接的免疫吸收剂测定法（ELISA）或 放射免疫测定（RIA）。因为某些测定可能无法商业化，因此此核心也将 使用商业上可用的抗体生成房屋ELISA和RIA方法。这个核心单元将 生成跨越SCCOR计划的所有项目的数据，因此应为 动物模型与人类疾病之间的比较。