ANIMAL CORE

动物核心

• 批准号: 7584075
• 负责人: William Edmund Sonntag
• 金额: $ 35.43万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7584075
• 关键词: Adult; Aging; Animal Model; Animals; Appendix; Behavioral; Biology of Aging; Blood flow; Body Weight; Brain; Breeding; Cannulas; Cardiovascular system; Collaborations; Condition; Confounding Factors (Epidemiology); Data; Disease; Doctor of Philosophy; Endocrine; Ensure; Family suidae; Female; Funding; Health; Hormonal; Hormones; Human Resources; Implant; Industry; Insulin-Like Growth Factor I; Investigation; Learning; Manuscripts; Measures; Memory; Modeling; Monitor; Norway; Operative Surgical Procedures; Oxidative Stress; Pathology; Peripheral; Plasma; Pump; Rattus; Research Personnel; Resources; Saline; Sentinel; Services; Somatotropin; Specific Pathogen Frees; Standards of Weights and Measures; Stress; Techniques; Telemetry; Time; Tissues; Validation; age related; aging brain; animal care; animal tissue; day; frontal lobe; germ free condition; growth hormone deficiency; irradiation; male; man; novel; programs; success

One of the primary factors in assessing specific mechanisms of brain aging is high quality animal care. One of the most common confounding variables in aging studies are inconsistent animal care, non-specific stress, and the presence of unrecognized age-related pathologies that compromise the dependent variables under investigation. For these reasons (and others), the Animal Core within the PPG must be of the highest quality. In the past funding period, this Core has been successful in maintaining animals in specific pathogen free (SPF) conditions for project studies, in developing and providing extensive validation of a novel model of adult-onset growth hormone deficiency that will continue to be used in the proposed funding period. This model provides a unique resource for the Program Project and opportunity to investigate the effects of growth hormone deficiency apart from other factors that may contribute to brain aging. The Animal Core has multiple aims: 1) The Core will procure and maintain animals used by program investigators and meet all AAALAC and institutional standards for animal care in the satellite facilities. The Core will also maintain a breeding colony of dw/+ (female) and dw/dw (male) Lewis rats and raise offspring to be used for the model of adult-onset growth hormone deficiency proposed in this application. The Core will coordinate animal/tissue use among the projects. 2) The Core personnel will assess basic parameters of aging animals and monitor sentinel and experimental animals for the presence of disease. 3) The Core will provide surgical services. 4) The Core will be responsible for irradiation of animals, as necessary, and measure hormone levels in tissue. 5) The Core will conduct behavioral analyses on animals to assess age-related alterations in hippocampally dependent measures of learning and memory and will develop frontal-lobe dependent tasks of learning and memory. The Animal Core is an essential aspect of the Program Project ensuring effective management of limited resources, smooth interactions between projects and the use of animal models that have been extensively validated for studies of the biology of aging.

评估脑老化特定机制的主要因素之一是高质量的动物护理。 衰老研究中最常见的混杂变量之一是不一致的动物护理，非特异性 压力，以及未识别的与年龄相关的病理，损害因变量 正在调查。由于这些原因（以及其他原因），PPG内的动物核心必须是最高的 质量。在过去的资金期间，该核心已成功地维持动物特定的动物 用于项目研究的病原体无病原体（SPF）条件，在开发和提供广泛的验证 成人发作生长激素缺乏症的新型模型，该模型将继续用于拟议的资金 时期。该模型为程序项目提供了独特的资源，并提供了调查的机会 除其他可能导致大脑衰老的因素外，生长激素缺乏症的影响。动物 核心具有多个目标：1）核心将采购和维护计划调查人员和 在卫星设施中符合所有AAAALAC和机构标准。核心也将 维持DW/+（雌性）和DW/DW（雄性）Lewis大鼠的繁殖菌落，并提高后代以用于 在本应用中提出的成年生长激素缺乏症的模型。核心将协调 项目之间的动物/组织使用。 2）核心人员将评估衰老动物的基本参数 并监测前哨和实验动物的疾病。 3）核心将提供手术 服务。 4）核心将在必要时负责动物的辐射，并测量激素 组织中的水平。 5）核心将对动物进行行为分析，以评估与年龄相关的变化 学习和记忆的海马依赖性措施，并将开发额叶依赖的任务 学习和记忆。动物核心是计划项目的重要方面，可确保有效 管理有限的资源，项目之间的平稳互动以及动物模型的使用 已对衰老生物学的研究进行了广泛的验证。