A germline- and promoter-independent strategy to gain access to all cell types in the brain

一种独立于种系和启动子的策略，可获取大脑中所有细胞类型

• 批准号: 10651435
• 负责人: HUAIYU HU
• 金额: $ 341.87万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10651435
• 关键词: 3' Untranslated Regions; Amaze; Anatomy; Animal Model; Attention; Brain; CRISPR/Cas technology; Callithrix; Capsid; Cell physiology; Cre driver; Cre lox recombination system; DNA; DNA sequencing; Dependovirus; Development; Electrophysiology (science); Enterobacteria phage P1 Cre recombinase; Fibroblasts; Gene Expression; Genes; Genetically Modified Animals; Goals; Guide RNA; Heterogeneity; High-Throughput Nucleotide Sequencing; Immune response; Immunohistochemistry; In Vitro; Internal Ribosome Entry Site; Knowledge; Libraries; Mediating; Methods; Modification; Molecular; Mus; Neurons; Nonhomologous DNA End Joining; Population; Primates; Process; Property; Reporter; Research; Specificity; System; Technology; Testing; Transgenic Animals; Vision; Visual Cortex; Work; brain cell; cell type; cost; design; experimental study; genome editing; improved; in vivo; mRNA delivery; multiplex assay; nervous system disorder; neural circuit; neuroinflammation; nonhuman primate; novel; promoter; recombinase; technology development; tool; transcriptome

Project summary The heterogeneity from the vast number of cell types in the brain presents a major challenge in our understanding of how brain works and in our treatment of neurological disorders. With the amazing advances in high throughput sequencing technology, our knowledge on the molecular makeup of the myriad cell types in the brain has reached an unprecedented level. However, tools that allow us to easily study the functions of any cell types of our choice are lagging. The goal of our proposed research is to develop technology to generate such tools. In order to target a specific cell type, current approaches typically depend on genetically modified animal models, which is laborious, costly, and low throughput, or well-defined and small cell-type-specific promoters, which still remain to be difficult to isolate. Here we propose to develop an AAV-based Cell-Type-Specific Targeting (ACTSTar) system to enable easy access to any cell type in the brain in any species that is independent of germline modification and promoter isolation and characterization. We plan to generate a library of ACTSTar drivers to target 50 different brain cell types in the mouse and marmoset. In addition, to reduce off-target integration and neuroinflammation associated with long-term Cas9 expression, especially in long-term experiments in primates, we will develop a novel AAV-capsid mediated Cas9 mRNA delivery system for transient Cas9 expression. This project will generate the much-needed tools for easy access to any brain cell types in multiple species. Our research goal will be facilitated by the complementary expertise of the MPI team in genome editing and developing safe Cas9 delivery methods (Lu), neural circuit functions of the mouse brain (Lin), and visual functions using non-human primates (Hu).

项目概要 大脑中大量细胞类型的异质性对我们的理解提出了重大挑战 大脑如何工作以及神经系统疾病的治疗。随着高通量方面的惊人进步 测序技术，我们对大脑中多种细胞类型的分子组成的了解已经 达到了前所未有的水平。然而，可以让我们轻松研究任何细胞类型功能的工具 我们的选择是滞后的。我们提出的研究的目标是开发生成此类工具的技术。在 为了针对特定的细胞类型，当前的方法通常依赖于转基因动物模型， 这是费力、昂贵且低通量的，或者是定义明确的小细胞类型特异性启动子，仍然 仍然难以隔离。在这里，我们建议开发一种基于 AAV 的细胞类型特异性靶向 （ACTSTar）系统能够轻松访问任何物种大脑中的任何细胞类型，独立于 种系修饰以及启动子分离和表征。我们计划生成一个 ACTSStar 库 驱动程序针对小鼠和狨猴体内 50 种不同的脑细胞类型。此外，为了减少脱靶 与长期 Cas9 表达相关的整合和神经炎症，尤其是长期表达 在灵长类动物实验中，我们将开发一种新型 AAV 衣壳介导的 Cas9 mRNA 递送系统，用于瞬时 Cas9表达。该项目将生成急需的工具，以便轻松访问任何脑细胞类型 多个物种。 MPI 团队在基因组方面的互补专业知识将促进我们的研究目标 编辑和开发安全的 Cas9 传递方法 (Lu)、小鼠大脑的神经回路功能 (Lin)，以及 使用非人类灵长类动物（Hu）的视觉功能。