NMR User Program at NMRFAM

NMRFAM 的 NMR 用户计划

• 批准号: 10647756
• 负责人: Samuel E Butcher
• 金额: $ 93.3万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10647756
• 关键词: Address; Adopted; Advisory Committees; Area; Automation; Biological; Biomedical Research; Biophysics; Calibration; Capital; Cell Nucleus; Collaborations; Communication; Communities; Complex; Computer software; Consult; Cryoelectron Microscopy; Data; Data Analyses; Data Collection; Data Security; Detection; Development; Doctor of Philosophy; Educational process of instructing; Educational workshop; Emerging Technologies; Ensure; Experimental Designs; FAIR principles; Goals; Helium; Home; Human Resources; Hybrids; Individual; Infrastructure; Institution; Isotopes; Knowledge; Ligands; Liquid substance; Magic; Magnetic Resonance; Maintenance; Membrane Proteins; Methods; Mission; Molecular Weight; NMR Spectroscopy; National Center for Research Resources; National Institute of General Medical Sciences; Natural Products; Nucleic Acids; Performance; Pharmaceutical Preparations; Phase; Physiologic pulse; Procedures; Proteins; Protocols documentation; Quality Control; R24; Recovery; Recycling; Reproducibility; Research Support; Resources; Sampling; Schedule; Scientist; Services; Site; Standardization; Structure; Surveys; System; Technology; Testing; Time; Training; Tube; Work; Writing; base; community engagement; computerized data processing; cryogenics; cyber security; data quality; data sharing; experience; experimental study; improved; instrument; instrumentation; interest; knowledge base; metabolomics; next generation; operation; outreach; programs; protein structure; recruit; repaired; screening; solid state nuclear magnetic resonance; structural biology; tool; training opportunity; web server

PROJECT SUMMARY The National Magnetic Resonance Facility at Madison (NMRFAM) provides timely and reliable access to mature NMR technology for application to a wide array of biomedical research questions. NMRFAM has staff with decades of experience in facility operation and maintenance, a dozen high-field NMR spectrometers equipped with state-of-the-art probe technology, and facility infrastructure (ample space, uninterrupted power systems, environmental control, helium recovery) second to none, and NMRFAM has been at the forefront of standardization, data stewardship through FAIR (findable, accessible, interoperable and reusable) data principles and dissemination of best practices to the community. Support through the NIGMS R24 program of NMRFAM through the R24 mechanism at NIGMS would support broad access to this exceptional resource in Madison to leverage economies of scale, including stable and efficient operation that maximizes data quality and minimizes instrument down time, facilitating many types of experiments that scientists are not able to perform at their home institutions. Furthermore, the proposed next generation user program improves the rigor and reproducibility of NMR data collection through instrument quality control, standard operating procedures, new sample standards for microcrystalline proteins and nucleic acids, membrane proteins and fibrils, including development of approaches for dynamics and function as well as structure. NMRFAM staff provide an exceptional level of service tailored to the needs of individual users and projects, serving arguably the broadest user base of any NMR facility in the US. NMRFAM actively seeks to support diverse users through outreach and user training opportunities (workshops, video tutorials, protocols) to teach users how to understand and analyze their NMR data. NMRFAM has a goal of providing users more than spectra, and the facility provides staff support and automation tools to return deliverables: i.e., scientific knowledge not only data. The NMRFAM mission lowers barriers to NMR and maximizes the impact of the facility when users can successfully apply the NMR data to answer their biological question of interest. NMRFAM has built a reputation as a top-level facility for external users and this project will maintain affordable access to NMRFAM. NMRFAM staff will regularly evaluate performance criteria and consult with the external advisory committee and facility users so that the facility continues to remain at the cutting edge and meet the needs of its user base.

项目概要 麦迪逊国家磁共振设施 (NMRFAM) 提供及时、可靠的成熟信息 NMR 技术适用于广泛的生物医学研究问题。 NMRFAM 的工作人员 数十年的设施运维经验，配备十几台高场核磁共振波谱仪 拥有最先进的探头技术和设施基础设施（充足的空间、不间断的电力系统、 环境控制、氦气回收）首屈一指，NMRFAM 一直处于领先地位 标准化，通过公平（可查找、可访问、可互操作和可重用）数据进行数据管理 原则并向社区传播最佳实践。通过 NIGMS R24 计划提供支持 NMRFAM 通过 NIGMS 的 R24 机制将支持广泛访问这一特殊资源 麦迪逊利用规模经济，包括稳定高效的运营，最大限度地提高数据质量和 最大限度地减少仪器停机时间，促进科学家无法进行的许多类型的实验 他们的家乡机构。此外，所提出的下一代用户程序提高了严谨性和 通过仪器质量控制、标准操作程序、新方法实现 NMR 数据采集的可重复性 微晶蛋白和核酸、膜蛋白和原纤维的样品标准品，包括 动力学、功能以及结构方法的开发。 NMRFAM 工作人员提供 根据个人用户和项目的需求量身定制卓越的服务水平，可以说服务于最广泛的人群 美国任何 NMR 设施的用户群。 NMRFAM 积极寻求通过外展支持多元化用户 以及用户培训机会（研讨会、视频教程、协议），教用户如何理解和 分析他们的核磁共振数据。 NMRFAM 的目标是为用户提供的不仅仅是光谱，而且该设施还提供 员工支持和自动化工具返回可交付成果：即科学知识而不仅仅是数据。 NMRFAM 当用户能够成功应用 NMR 时，任务会降低 NMR 的障碍并最大限度地发挥设施的影响 核磁共振数据可以回答他们感兴趣的生物学问题。 NMRFAM 已建立了顶级设施的声誉 对于外部用户来说，该项目将保持对 NMRFAM 的经济访问。 NMRFAM 工作人员会定期 评估绩效标准并与外部咨询委员会和设施用户协商，以便 设施继续保持领先地位并满足其用户群的需求。

项目成果

Rapid Unambiguous Structure Elucidation of Streptnatamide A, a New Cyclic Peptide Isolated from A Marine-derived Streptomyces sp.

快速明确的结构阐明链酰胺 A，一种从海洋来源的链霉菌中分离出来的新型环肽。

• 发表时间: 2023-10-09
• 作者: Yan, Jia;Wu, Qihao;Maity, Mitasree;Braun, Doug R;Alas, Imraan;Wang, Xiao;Yin, Xing;Zhu, Yanlong;Bell, Bailey A;Rajski, Scott R;Ge, Ying;Richardson, Douglas D;Zhong, Wendy;Bugni, Tim S
• 通讯作者: Bugni, Tim S

A platform for the rapid synthesis of proteolysis targeting chimeras (Rapid-TAC) under miniaturized conditions.

在小型化条件下快速合成蛋白水解靶向嵌合体（Rapid-TAC）的平台。

• DOI: 10.1016/j.ejmech.2022.114317
• 发表时间: 2022-06-05
• 期刊: European journal of medicinal chemistry
• 影响因子: 6.7
• 作者: Guo, Le;Zhou, Yaxian;Nie, Xueqing;Zhang, Zhongrui;Zhang, Zhen;Li, Chunrong;Wang, Taobo;Tang, Weiping
• 通讯作者: Tang, Weiping

Activating alternative transport modes in a multidrug resistance efflux pump to confer chemical susceptibility.

激活多药耐药性外排泵中的替代运输模式以赋予化学敏感性。

• 发表时间: 2022-12-10
• 影响因子: 16.6
• 作者: Spreacker, Peyton J;Thomas, Nathan E;Beeninga, Will F;Brousseau, Merissa;Porter, Colin J;Hibbs, Kylie M;Henzler
• 通讯作者: Henzler

Rapid Biocatalytic Synthesis of Aromatic Acid CoA Thioesters by Using Microbial Aromatic Acid CoA Ligases.

使用微生物芳香酸 CoA 连接酶快速生物催化合成芳香酸 CoA 硫酯。

• 发表时间: 2023-05-02
• 作者: Chaudhury, Debayan;Torkelson, Ella R;Meyers, Kaya A;Acheson, Justin F;Landucci, Leta;Pu, Yucen;Sun, Zimou;Tonelli, Marco;Bingman, Craig A;Smith, Rebecca A;Karlen, Steven D;Mansfield, Shawn D;Ralph, John;Fox, Brian G
• 通讯作者: Fox, Brian G

The mechanism of RNA capping by SARS-CoV-2.

SARS-CoV-2 对 RNA 加帽的机制。

• 发表时间: 2022-09
• 影响因子: 64.8
• 作者: Park, Gina J;Osinski, Adam;Hernandez, Genaro;Eitson, Jennifer L;Majumdar, Abir;Tonelli, Marco;Henzler;Pawłowski, Krzysztof;Chen, Zhe;Li, Yang;Schoggins, John W;Tagliabracci, Vincent S
• 通讯作者: Tagliabracci, Vincent S