NMR OF U6 RNA

U6 RNA 的 NMR

• 批准号: 8361218
• 负责人: Samuel E Butcher
• 金额: $ 0.23万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361218
• 关键词: Binding Proteins; Funding; Grant; Label; National Center for Research Resources; Principal Investigator; Procedures; Proteins; Research; Research Infrastructure; Resources; Sampling; Source; Structure; Time; U6 small nuclear RNA; United States National Institutes of Health; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. NMR time is requested to investigate the structure of a U6 RNA binding protein, Prp24. This protein is 25 kDa and well folded as determined by 2D HSQC. We intend to solve the structure of Prp24, and have produced a 13C, 15N double labeled NMR sample for this purpose. Assignments will be obtained through an automated assignment procedure, PISTACHIO, recently developed by Eghbalnia et al.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 需要 NMR 时间来研究 U6 RNA 结合蛋白 Prp24 的结构。 该蛋白质为 25 kDa，经 2D HSQC 测定折叠良好。 我们打算解析Prp24的结构，并为此目的制备了13C、15N双标记NMR样品。 分配将通过自动分配程序 PISTACHIO 获得，该程序最近由 Eghbalnia 等人开发。