CORE--IN VIVO FUNCTIONALITY

核心——体内功能

• 批准号: 7892320
• 负责人: JOSEPH Mark METZGER
• 金额: $ 15.74万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7892320
• 关键词: Acute; Affect; Age; Aging; Animals; Arts; Basic Science; Biology of Aging; Blood Pressure; Cardiac; Catheters; Collection; Complex; Core Facility; Data; Disease; Electrocardiogram; Engineering; Equipment; Exercise; Faculty; Food; Genes; Genetic; Goals; Human Engineering; Individual; Inherited; Laboratories; Left; Manuscripts; Measurement; Measures; Metabolic; Michigan; Molecular Biology; Monitor; Mus; Operative Surgical Procedures; Performance; Phenotype; Physiological; Physiological Processes; Physiology; Positioning Attribute; Procedures; Recovery; Research; Research Personnel; Rodent; Services; Shock; Stress; System; Techniques; Technology; Time; Tissues; Training; Universities; Ventricular; Whole Organism; age effect; animal old age; body system; computerized; data integration; drinking; experience; gene environment interaction; implantation; in vivo; instrumentation; longitudinal analysis; member; nutrition; research study; retinal rods

The In Vivo Functionality Core is directed by Joseph Metzger. The major purpose of this core is to provide Nathan Shock Center (NSC) faculty with state-of-the art facilities and technical assistance in the in vivo physiological assessments of the aging rodent. This Core is uniquely positioned to offer NSC faculty members facilities and expert training for understanding the complex and adaptive gene-environment interactions in animals, including as examples, disease genes, genetic modifiers, nutrition, exercise, and stress, with a focus on aging and old animals. The Core facilities are fully operational, and over the past year the Core has provided services to 14 NSC faculty members. Cores include state-of-the-art equipment for high fidelity in vivo assessment of function such as, computerized food and drink monitoring system, metabolic chambers, six-lane treadmill, and Rota-rod. Also available are high thru-put analysis of blood pressure and EKG, as well as more precise and time-intensive instrumentation such as, in-vivo assessment of left ventricular cardiac performance by micromanometry catheters. Training and assistance are available for Core components. A surgical laboratory provides a convenient facility for researchers to perform surgical procedures on rodents. The Core has expert training in the operation of these platforms and also provides basic surgical techniques if needed. The Specific Aims are: Aim 1. To provide state-of-the-art facilities and instrumentation for the in vivo functional assessment of young and old mice, as well as to provide the necessary training in the use of the facilities and in the analysis of these highly complex data to Nathan Shock Center faculty who are determining the effects of aging on whole animal function. The Core will provide: (a) well-maintained, complex, state-of-the-art equipment, (b) training and assistance in the collection of reliable and valid data on appropriate measures of whole animal physiology, (c) analysis and presentation of the data, and (d) assistance with mechanistic interpretations of the data and integration of the interpretations into the manuscript. Aim 2. To provide expert training in rodent surgery procedures, instrumentation, and implantation, including both acute and recovery procedures, for the assessment of in vivo function in young and old animals.

体内功能核心由约瑟夫·梅茨格（Joseph Metzger）指导。该核心的主要目的是为内森冲击中心（NSC）教师提供最先进的设施，并在体内生理评估中对衰老啮齿动物进行技术援助。该核心具有独特的位置，可以为NSC教职员工设施和专家培训提供理解动物中复杂和适应性的基因环境相互作用，包括作为例子，疾病基因，遗传修饰符，营养，运动和压力，重点是衰老和老动物。核心设施是完全运营的，在过去的一年中，核心为14名NSC教职员工提供了服务。核心包括用于体内高保真的最先进设备 评估功能，例如计算机食品和饮料监测系统，代谢室，六车道跑步机和旋转rod。还提供了对血压和心电图的高度直通分析，以及更精确，更耗时的仪器，例如，通过微观管理导管对左心室心脏性能的体内评估。培训和帮助可用于核心组件。外科实验室为研究人员提供了方便的设施，可以对啮齿动物进行手术程序。核心对这些平台的运行进行了专家培训，并在需要时还提供基本的手术技术。具体目的是：目标1。为IN提供最新的设施和工具 体内功能评估是对年轻小鼠的体内功能评估，以及为设施使用的必要培训，以及分析这些高度复杂的数据向Nathan Shock Center的教职员工分析，他们正在确定衰老对整个动物功能的影响。核心将提供：（a）良好，复杂，最先进的设备，（b）在收集可靠和有效数据的整个动物生理学测量中，（c）数据的分析和介绍，以及（d）解释解释的机械解释和解释的机械解释。目的2。提供啮齿动物手术程序，仪器和植入（包括急性和恢复程序）的专家培训，以评估年轻动物和老年动物体内功能。