Protein Core

蛋白质核心

• 批准号: 7918817
• 负责人: John Atkinson
• 金额: $ 20.12万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7918817
• 关键词: B-Lymphocytes; Core Protein; Disease model; Funding; Generations; Hybridomas; Laboratories; Monoclonal Antibodies; Operative Surgical Procedures; Production; Proteins; Reagent; Recombinant Fusion Proteins; Recombinant Proteins; Research; Research Personnel; Rheumatism; Role; Services; Source; Technology; Testing; Training; biomedical scientist; cost; interest; protein function; scale up; success; B-Lymphocytes; Core Protein; Disease model; Funding; Generations; Hybridomas; Laboratories; Monoclonal Antibodies; Operative Surgical Procedures; Production; Proteins; Reagent; Recombinant Fusion Proteins; Recombinant Proteins; Research; Research Personnel; Rheumatism; Role; Services; Source; Technology; Testing; Training; biomedical scientist; cost; interest; protein function; scale up; success

To analyze a protein of interest, it is necessary to develop reagents that can detect it. Furthermore, to investigate the role of a protein in rheumatic disease models, it is frequently desirable to use monoclonal antibodies (mAbs) or recombinant proteins that block the function of the protein in question. A Protein Core has been established and has been productive during the last funding period. The RDCC proposes to continue support of this facility to provide services through a 1) Hybridoma facility to produce new mAbs; and 2) Production and Purification facility to produce high quality purified mAbs or recombinant proteins of interest. New services are planned to enhance the facility. Thus, the facility will continue to provide investigators with large quantities of proteins that can be used to analyze their molecules in relationship to rheumatic disease models.

为了分析感兴趣的蛋白质，有必要开发可以检测到它的试剂。此外， 研究蛋白质在风湿病模型中的作用，通常希望使用单克隆 阻断所讨论蛋白功能的抗体（mAb）或重组蛋白。蛋白质核心 已建立并在最后一个资金期间一直是富有成效的。 RDCC建议 继续支持该设施，以通过A）1）杂交瘤设施生产新的mAB；和 2）生产和纯化设施，以生产高质量纯化的单克隆抗体或重组蛋白 兴趣。计划新服务来增强设施。因此，该设施将继续提供 大量蛋白质的研究者可用于分析其分子与 风湿病模型。