Protein Core

蛋白质核心

• 批准号: 8379367
• 负责人: John Atkinson
• 金额: $ 18.26万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8379367
• 关键词: Communities; Core Facility; Core Protein; Generations; Growth; Hybridomas; Immunology; Monoclonal Antibodies; Oryctolagus cuniculus; Production; Proteins; Recombinants; Research; Research Personnel; Rheumatism; Services; System; Testing; Universities; Washington; genetic immunization strategies; member

The Protein Core facility is one of the research cores in the Rheumatic Diseases Core Center (RDCC) at Washington University. It proposes the following Specific Aims to sustain and develop new state-of-the-art protein services to RDCC members: 1) Support continued progress and growth of the Hybridoma Center; and 2) Support continued progress and growth of the Protein Production and Purification Facility. The Hybridoma Center aids RDCC investigators in the generation of primary hybridomas secreting monoclonal antibodies that are critical to contemporary immunology and rheumatic diseases research. New services are being tested to offer RDCC investigators rabbit monoclonal antibodies and genetic immunization, for example. The Protein Production and Purification Facility aids RDCC investigators by producing and purifying large quantities of monoclonal antibodies for their studies. In addition, it has recently gained expertise in producing and purifying recombinant and native proteins from a number of different expression systems. This service will now be made available to the RDCC community.

蛋白质核心设施是华盛顿大学风湿病核心中心（RDCC）的研究核心之一。它提出了以下特定旨在维持和开发新的最先进蛋白质服务的RDCC成员：1）支持杂交瘤中心的持续进展和增长； 2）支持蛋白质生产和纯化设施的持续进展和增长。杂交瘤中心有助于RDCC研究人员产生原发性杂交瘤，分泌单克隆抗体，这些抗体对当代免疫学和风湿病研究至关重要。例如，正在测试新服务，以提供RDCC调查人员兔子单克隆抗体和遗传免疫。蛋白质生产和纯化设施通过生产和纯化大量的单克隆抗体进行研究来有助于RDCC研究者。此外，它最近从许多不同表达系统中生产和净化重组和天然蛋白质方面获得了专业知识。现在，该服务将提供给RDCC社区。